We narrowed to 3,483 results for: mpo

1. pAAV-hSyn-DIO-GRAB_eCB2.0mut

   Plasmid

   #164607

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Use

   AAV

   Mutation

   S383T, F177A

   Promoter

   hSyn

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. (SUMO)10-(SIM)5

   Plasmid

   #126866

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP Lamp1-Venus

   Plasmid

   #89937

   Purpose

   Expresses Lamp1 tagged with Venus in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

   Insert

   rat Lamp1 (Lamp1 Rat)
   

   Use

   Retroviral

   Tags

   Venus

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-MCU

   Plasmid

   #31726

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Mutation

   *

   Promoter

   None

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

   Plasmid

   #174884

   Purpose

   Targeting vector for generating Sox2 STREAMING-tag KI cell

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   STREAMING-tag with homology arms for Sox2
   

   Use

   Mouse Targeting

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5HT6-YFP-ThymosinBeta4(WT)

   Plasmid

   #96806

   Purpose

   cilia-membrane-targeted G-actin-binding protein

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR357.24

   Plasmid

   #125109

   Purpose

   Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-ydfI(DBD)159
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-GFP-AURKA

   Plasmid

   #99875

   Purpose

   Expression of GFP-AURKA for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AE-Polyubiquitin-GCaMP6s

    Plasmid

    #106868

    Purpose

    Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

    Depositor

    Omar Akbari

    Article

    Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

    Insert

    AE-Polyubiquitin-GCAaMP6s
    

    Expression

    Insect

    Promoter

    Ae. aegypti polyubiquitin (PUb)

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5HT6-YFP-ThymosinBeta4(KK18,19EE)

    Plasmid

    #96807

    Purpose

    non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    KK18,19EE prevents G-actin binding

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGH10 - RlucFluc minus 1 HIV-1

    Plasmid

    #198224

    Purpose

    Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

    Depositor

    Isha Jain

    Article

    Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKII-GRAB_eCB2.0

    Plasmid

    #164608

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    CaMKII

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUBC_HA_PP7-mVenus

    Plasmid

    #111268

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mVenus
    

    Use

    Synthetic Biology

    Tags

    HA, SV40 NLS, and mVenus

    Expression

    Mammalian

    Mutation

    PP7: C68S, S69G [delta]70-75 (non-aggregating)

    Promoter

    hUBC

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_pSyn_Mito-LOV-Turbo-V5

    Plasmid

    #199669

    Purpose

    expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    COX4I1 mito targeting sequence and V5

    Promoter

    Synapsin

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FKBP-alpha(740-977)-GFP

    Plasmid

    #100731

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2A1 (Ap2a1 Mouse)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEX-Antenna-GDI RhoA

    Plasmid

    #189669

    Purpose

    Expression of Antenna-RhoA

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    RhoA (RHOA Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mTcte1-PA

    Plasmid

    #176469

    Purpose

    Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    t-complex-associated testis expressed 1 (Tcte1 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRESneo-Ago2

    Plasmid

    #215841

    Purpose

    Expression of AGO2

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    Human AGO2 (AGO2 Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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