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pBabe H-Ras 12V

Plasmid

#12545

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

H-Ras 12V (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated.

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-RH

Plasmid

#60516

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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Hsp68-CreER(TM) (DM#264)

Plasmid

#15028

Depositor

Douglas Melton

Insert

Cre-ER(TM)

Use

Cre/Lox; For making transgenic constructs

Tags

Hsp68 promoter and polyA

Expression

Mammalian

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pRC-CMV-Rev1b

Plasmid

#164443

Purpose

Encodes HIV-1 Rev accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Rev

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLX302 Luciferase-V5 puro

Plasmid

#47553

Purpose

Lentiviral expression of V5-tagged Luciferase in mammalian cells

Depositor

Kevin Janes

Article

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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Monomer NH_V2

Plasmid

#37527

Purpose

template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

Monomer NH

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHR SFFVp Phy-mCherry-CAAX

Plasmid

#50839

Purpose

Optogenetic PI3K activation system; membrane component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PhyB 1-908

Use

Lentiviral and Synthetic Biology

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-ASH1L

Plasmid

#65369

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ASH1L (ASH1L Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3A2

Plasmid

#36941

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3A2 (DNMT3A Human)

Tags

Myc

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ421 - Pmyo-2::GFP::H2B (pharynx)

Plasmid

#34876

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Pmyo-2 GFP H2B tbb-2 UTR

Use

Worm targeting

Mutation

S65C

Promoter

myo-2

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84239

Purpose

Expresses ABA-inducible VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-B/c

Plasmid

#51776

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Article

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Insert

ERK2 (MAPK1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
CAG-NeuroD1iresGFP

Plasmid

#45025

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD1 (Neurod1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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