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pGEM-and4 in situ probe

Plasmid

#52105

Purpose

actinodin 4 and4 antisense in situ probe linearize w/ AatII SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 4 in situ probe (and4 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and2 in situ probe

Plasmid

#52103

Purpose

actinodin 2 and2 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 2 in situ probe (and2 Zebrafish)

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Expression

Bacterial

Promoter

Ptb38

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-767-Decoy.hPGK.GFP.WPRE

Plasmid

#46636

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-767 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-299-Decoy.hPGK.GFP.WPRE

Plasmid

#46604

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-299 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-323-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46609

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-323-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-337-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46613

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-337-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-339-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46614

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-339-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-345-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46615

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-345-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-369-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46616

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-369-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-409-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46619

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-409-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-489-Decoy.hPGK.GFP.WPRE

Plasmid

#46621

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-489 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-505-Decoy.hPGK.GFP.WPRE

Plasmid

#46623

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-505 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-532-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46624

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-532-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-574-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46625

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-574-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-582-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46626

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-582-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-582-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46627

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-582-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-654-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46628

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-654-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-668-Decoy.hPGK.GFP.WPRE

Plasmid

#46629

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-668 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-151-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46591

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-151-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-151-5p-Decoy.hPGK.GFP.WPRE

Plasmid

#46592

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-151-5p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_hcRED

Plasmid

#45286

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

hcRed

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_hcRED

Plasmid

#45458

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

hcRED

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-CGI12

Plasmid

#34800

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

CGI-12 (MTERF3 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-SUHW4

Plasmid

#34807

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

suppressor of hairy wing homolog 4 (Drosophila) (ZNF280D Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQStrep2-UBL3

Plasmid

#31330

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ubiquitin-like 3 (UBL3 Human)

Tags

His, StrepII-tag, and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-DKFZP566E144

Plasmid

#31593

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

small fragment nuclease (REXO2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-DGCR6

Plasmid

#31564

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

DiGeorge syndrome critical region gene 6 (DGCR6 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-hygro-mTurquoise2-Nuf2

Plasmid

#80762

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN432 (FuGW-G14p-mKate2)

Plasmid

#105187

Purpose

Module 3 - GAL4 synthetic promoter( with fourteen binding sites) drives mKate2

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G14p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

Enlarge
pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

Plasmid

#71760

Purpose

mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

Depositor

Liqun Luo

Article

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Has Service

AAV1

Inserts

mGFP
Synaptophysin

Use

AAV and Cre/Lox

Tags

Palmitoylation signal and mRuby

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209655

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Has Service

AAV5

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTSin EGFP

Plasmid

#127695

Purpose

Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

EGFP

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MS2-HB

Plasmid

#35573

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

MS2 Coat Protein (cp Bacteriophage)

Use

Retroviral

Tags

HTBH

Expression

Mammalian

Mutation

Insert is a tail-to-head tandem placement of two …

Promoter

CMV

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-LSSmApple

Plasmid

#191259

Purpose

Bacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple

Depositor

Robert Campbell

Article

Ejike et al Biochemistry. 2024 Jan 2;63(1):171-180.

Insert

LSSmApple

Tags

His tag

Expression

Bacterial

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK-Neo

Plasmid

#25735

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-ChRger2-TS-EYFP

Plasmid

#127237

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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