We narrowed to 3,857 results for: SPL;

1. sABE v3.22 Lenti_2

   Plasmid

   #209556

   Purpose

   pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pU6, pCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAtetO_6xHis-v5.9

   Plasmid

   #155259

   Purpose

   Plasmid for the aTc-inducible expression of the v5.9

   Depositor

   Andrew Ellington

   Article

   Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

   Insert

   v5.9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tet PA

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. tol2-CAG::ChR2-YFP

   Plasmid

   #59740

   Purpose

   Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   Channelrhodopsin-2
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CAG

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pACUH-sfcherry2(1-10)

   Plasmid

   #172061

   Purpose

   UAS vector to express sfcherry2(1-10) in Drosophila

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   sfcherry2(1-10)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TetO-U2B''-fSNAPc

   Plasmid

   #106988

   Purpose

   open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   UB2" (SNRPB2 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

   Plasmid

   #153008

   Purpose

   Expresses glucagon peptide on mammalian cell surface

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   Glucagon-ICAM1-pre-mGRASP
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PEP110E

   Plasmid

   #97396

   Purpose

   binary construct of nuclear mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   Nucleus target

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-SUN1_888

   Plasmid

   #125851

   Purpose

   Expresses SUN1_888 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_888 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   longCaMV35S

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

    Plasmid

    #131346

    Purpose

    gRNA targeting mouse TRAF6

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    gRNA targeting mouse TRAF6 (Traf6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC051-HisMS2_PLP_pac_LC

    Plasmid

    #128234

    Purpose

    Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

    Depositor

    Paul Freemont

    Article

    de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.1 FLIPE-1m

    Plasmid

    #13544

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CamKIIa-moxBFP-IRES-Flpo

    Plasmid

    #194977

    Purpose

    AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP, Flpo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-IRES-GFP-3xFLAG-MyD88-V5

    Plasmid

    #131351

    Purpose

    Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88 (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSiMPla_N + pSiMPla_C

    Plasmid

    #134314

    Purpose

    Maintenance of 2 plasmids with a single antibiotic in E. coli

    Depositor

    Barbara Di Ventura

    Article

    Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

    Insert

    ampR_gp41-1 N-intein + ampR_gp41-1 C-intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV/MASC_(GLuc)_TOP1

    Plasmid

    #68439

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PEP112E

    Plasmid

    #97398

    Purpose

    binary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Mitochondria target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NIR-AG

    Plasmid

    #210728

    Purpose

    NIR light-activated GSDMD

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    NIR-AG (GSDMD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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