We narrowed to 22,792 results for: Sis

1. pKIF1C-2xFLAG

   Plasmid

   #130979

   Purpose

   Expression of KIF1C-2xFLAG in mammalian cells.

   Depositor

   Anne Straube

   Article

   Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

   Insert

   KIF1C-2xFLAG (KIF1C Human)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   4 silent mutations that make this construct resis…

   Promoter

   CMV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgGria3

   Plasmid

   #124869

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPER-retro-puro-tetO-Mek1-shRNA

   Plasmid

   #53179

   Purpose

   Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (Map2k1 Mouse)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAN19-3xFLAG-TIR1-NOSt

   Plasmid

   #108545

   Purpose

   Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

   Depositor

   Keiko Torii

   Article

   Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
   

   Use

   Cloning vector

   Tags

   3xFLAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gal4-VP16-HSF1Δ∼PRD

   Plasmid

   #71727

   Purpose

   plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Budding Yeast, Human, Herpes Simplex Virus protein VP16)
   

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEK42

   Plasmid

   #214958

   Purpose

   Endogenous tagging of B. dendrobatidis GAPDH with a red fluorescent protein, hygromycin B selection

   Depositor

   Lillian Fritz-Laylin

   Article

   Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

   Inserts

   BdGAPDH_LHA2-G4S-HsmRuby3-ScADH1ter
   SpH2Bpro-Hshph-BdGAPDH_RHA2
   

   Use

   Bd gene targeting vector

   Expression

   Bacterial

   Promoter

   SpH2Bpro

   Available Since

   Oct. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEK48

   Plasmid

   #214964

   Purpose

   Endogenous tagging of B. dendrobatidis Myo17D with a red fluorescent protein, hygromycin B selection

   Depositor

   Lillian Fritz-Laylin

   Article

   Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

   Inserts

   BdMyo17D_LHA-G4S-HsmRuby3-ScADH1ter
   SpH2Bpro-Hshph-BdMyo17D_RHA
   

   Use

   Bd gene targeting vector

   Expression

   Bacterial

   Promoter

   SpH2Bpro

   Available Since

   Oct. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS

   Plasmid

   #241915

   Purpose

   Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

   Depositor

   Balázs Enyedi

   Article

   Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

   Insert

   QUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
   

   Use

   Zebrafish tol2 transgenesis

   Promoter

   QUASM1

   Available Since

   Oct. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIDTSmart-CMV-PLRS1

   Plasmid

   #217365

   Purpose

   Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   leucyl-tRNA synthetase polyspecific mutant
   

   Expression

   Mammalian

   Mutation

   M40I, T252A, Y499I, Y527A, H529G

   Promoter

   CMV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MT2

    Plasmid

    #223330

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
    

    Tags

    3HA-TurboID-EGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-HA-CBS(opt)-FLAG

    Plasmid

    #230088

    Purpose

    expressing human CBS

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    CBS (CBS Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. MT3

    Plasmid

    #223331

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
    

    Tags

    3HA-EGFP-miniTurboID

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MT4

    Plasmid

    #223332

    Purpose

    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
    

    Tags

    3HA-miniTurboID-EGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSuper Retro GFP DGK zeta human

    Plasmid

    #224579

    Purpose

    To stably downregulate the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Torres-Ayuso et al Oncogenesis. 2015 Aug 24;4(8):e164. doi:

    Insert

    sh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)
    

    Use

    RNAi and Retroviral

    Promoter

    H1

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1BR9-AtWUSSTOP-Δα3

    Plasmid

    #202056

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    third helix of homeodomain deleted (AA82-102)

    Promoter

    T7 Promoter

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNOC_hfnCas12a_crRNA NR2

    Plasmid

    #176248

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNOC_hfnCas12a_crRNA NR1

    Plasmid

    #176247

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNOC_hfnCas12a_crRNA NR3

    Plasmid

    #176249

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid

    #153445

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid

    #153444

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG). Argi…

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAN19-TIR1-GUS-NOSt

    Plasmid

    #108547

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Mutation

    Changed F79 to G

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMCh-1522

    Plasmid

    #82428

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    EF1A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMCh-1526

    Plasmid

    #82429

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWZLblasti-HA-MEK5 CBM-E

    Plasmid

    #53169

    Purpose

    Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L280A/H281A/M323A/Q332A

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

31. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7 promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pINDUCER21-PGBD5

    Plasmid

    #78121

    Purpose

    Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid

    #60367

    Purpose

    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI-D10R-E48R
    

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Mutation

    D10R+E48R; catalyticaly inactive

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CIBN-GFP-Sec61B

    Plasmid

    #104177

    Purpose

    Expresses fusion of CIB1 (1-170) with GFP and Sec61B

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CIBN-GFP-Sec61B (CIB1, SEC61B Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hTph2p-Luc

    Plasmid

    #223526

    Purpose

    Firefly-luciferase reporter expression driven by human Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Tph2 promoter (TPH2 Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Promoter

    hTph2 promoter

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

    Plasmid

    #231007

    Purpose

    Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    E4F1 aa220-242 (E4F1 Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBT272_(pRosa26-GTET)

    Plasmid

    #36882

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    diphteria toxin A
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge