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We narrowed to 4,881 results for: phy

Showing: 3841 - 3880 of 4881 results

  1. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  8. ORAI1-YFP T184M

    Plasmid
    #114181
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114181-plasmid-map-sequence-id-218676

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  19. p-OiRS3GG

    Plasmid
    #98589
    Purpose
    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
    Depositor
    Lydia Contreras
    Article
    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552
    Inserts
    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD and pLtetO
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    98589-plasmid-map-sequence-id-194130

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  20. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  21. FLAG-Cerulean-UCR1

    Plasmid
    #67578
    Purpose
    Expresses the UCR1 region of PDE4D3 in mammalian cells
    Depositor
    Graeme Carnegie
    Article
    Wang et al Cell Signal. 2015 May;27(5):908-22. doi:
    Insert
    Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
    Use
    Tags
    Cerulean and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid residues 17-136 of PDE4D3
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. 6xHsa.NFKB:Kaede

    Plasmid
    #239992
    Purpose
    Kaede driven by NFkB activity can be switched from green to red fluorescence by UV light exposure, enabling tracking of NFkB positive cells
    Depositor
    Raquel Espin-Palazon
    Insert
    photo convertible Kaede
    Use
    Tags
    NA
    Expression
    Mutation
    Promoter
    cfos minimal promoter
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239992-plasmid-map-sequence-id-477299

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  26. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Use
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

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  39. pRSF-G1TMSNKRS

    Plasmid
    #198323
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198323-plasmid-map-sequence-id-393448

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Showing: 3841 - 3880 of 4881 results