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pBiT3.1-N_WDR31:1-367

Plasmid

#211252

Purpose

Mammalian expression of full-length human WDR31 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

WDR31:M1-F367 (WDR31 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. 40-40: Missing

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:ffluc

Plasmid

#88900

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + firefly luciferase

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGST1-yGGA2-VHS

Plasmid

#44422

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA2 (1-169) (GGA2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pBaseline-PRM-wasabi

Plasmid

#195560

Purpose

Always-off, unactivated expression of mWasabi reporter

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Insert

mWasabi

Use

Tags

AANDENYADAS ssrA tag

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW12:1-394

Plasmid

#211208

Purpose

Mammalian expression of full-length human FBXW12 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW12 :M1-T394 (FBXW12 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW10:1-1061

Plasmid

#211207

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW12:1-394

Plasmid

#211282

Purpose

Mammalian expression of full-length human FBXW12 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW12 :M1-T394 (FBXW12 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW10:1-1061

Plasmid

#211281

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Use

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-V5

Plasmid

#180413

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72cCTLD

Plasmid

#129066

Purpose

generation of biotinylated mouse CD72c CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72c (Cd72 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Use

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-3

Plasmid

#180393

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-3 (Stk16 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET28aAviHisCD72aCTLD

Plasmid

#129065

Purpose

generation of biotinylated mouse CD72a CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72a (Cd72 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Gi2-CASE

Plasmid

#168121

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)

Use

Luciferase

Tags

cpVenus on GNG2

Expression

Mammalian

Mutation

NLuc is inserted at C112/E115 within GNAI2

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

rrnB P1

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast HA-POLE2

Plasmid

#160809

Purpose

Express HA-tagged POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

HA-POLE2 (POLE2 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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