163,584 results

1. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   no

   Promoter

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemon in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX_307

   Plasmid

   #41392

   Purpose

   Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-EGFP-C4-Nrf2

   Plasmid

   #21549

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV-Mito4x-GCaMP6f

   Plasmid

   #127870

   Purpose

   Fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   4 repeats of COX8 signal peptide plus GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-20nm-EBFP2

   Plasmid

   #231185

   Purpose

   Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   20nm-EBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDH-CMV-mCherry-T2A-Puro

    Plasmid

    #72264

    Purpose

    Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

    Depositor

    Kazuhiro Oka

    Insert

    mCherry-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEmerald-Nesprin3-C-18

    Plasmid

    #54203

    Purpose

    Localization: Nuclear Membrane , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Nesprin3 (SYNE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pZT-C13-R1

    Plasmid

    #62197

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Right CLYBL TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti CMV GFP Neo (657-2)

    Plasmid

    #17447

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AICSDP-7:SEC61B-mEGFP

    Plasmid

    #87426

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mScarlet-Rab7

    Plasmid

    #169068

    Purpose

    Express human Rab7a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TUBB5-Halo

    Plasmid

    #64691

    Purpose

    Expresses beta-tubulin labeled with Halo-tag

    Depositor

    Yasushi Okada

    Article

    Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

    Insert

    TUBB
    

    Use

    Tags

    Halo-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_PGK_antiCD19_synNotch_Gal4VP64

    Plasmid

    #79125

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-DJ-N589X

    Plasmid

    #130878

    Purpose

    Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    AAV-DJ cap N589X
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N589X

    Promoter

    Unknown

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge