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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Publication

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Publication

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Publication

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pGP-CMV-GCaMP6s-CAAX

Plasmid

#52228

Purpose

Plasma membrane targeted GCaMP6s, calcium sensor

Depositor

Tobias Meyer

Publication

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

GCaMP6s-CAAX

Tags

6xHis and CAAX

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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3XMEF2-luc

Plasmid

#32967

Purpose

Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

Depositor

Ron Prywes

Insert

MEF2 binding sites (MEF2A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

MEF2

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pcDNA3.1 - HA-KLF4 FL

Plasmid

#34593

Depositor

Michael Ruppert

Publication

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMedium-FT-N1

Plasmid

#31911

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Medium-FT

Expression

Mammalian

Mutation

M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-eNpHR 3.0-EYFP

Plasmid

#26972

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Has service

AAV2 and AAV5

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

hSyn

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pcDNA4-Beclin1-HA

Plasmid

#24399

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

HA

Expression

Mammalian

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-USP15

Plasmid

#22570

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP15 (USP15 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti CMV/TO Neo DEST (685-3)

Plasmid

#17292

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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IgK-IFN-luc

Plasmid

#14886

Purpose

Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

Depositor

David Baltimore

Publication

Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

Insert

IgK-IFN (IFNB1 Human)

Use

Luciferase

Expression

Mammalian

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAKlux2

Plasmid

#14080

Depositor

Attila Karsi

Publication

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Promoter

Lac, T3

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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SIRT7 Flag

Plasmid

#13818

Purpose

Mammalian expression of human SIRT7 with flag tag

Depositor

Eric Verdin

Publication

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT7 (SIRT7 Human)

Tags

flag

Expression

Mammalian

Mutation

Missing exon 2

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-hAgo2

Plasmid

#11590

Depositor

Edward Chan

Publication

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

Ago2 (AGO2 Human)

Tags

GFP

Expression

Mammalian

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Publication

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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