We narrowed to 7,580 results for: Ski;

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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SNAP-OMP25

Plasmid

#141149

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat, Budding Yeast)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

Plasmid

#217686

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2516

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-FGFR1

Plasmid

#222611

Purpose

Expression of MAC3-tagged FGFR1

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

FGFR1 (FGFR1 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ10875

Plasmid

#183963

Purpose

CBA-hyperdCas12a-HA-KRAB-mCherry

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, KRAB, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CBA

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Sec61B

Plasmid

#141152

Purpose

Fluorescent labeling of ER membrane in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Sec61 beta (SEC61B Human)

Tags

SNAP tag

Expression

Mammalian

Mutation

L79M on Sec61B

Promoter

CMV

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCEFL EGFP-TEADi

Plasmid

#140144

Purpose

TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-C2-SNAP

Plasmid

#208769

Purpose

To express C2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

Plasmid

#169333

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 YFP

Plasmid

#84910

Purpose

expression of YFP in mammalian cells

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTagRFP658-N1

Plasmid

#178974

Purpose

Expresses TagRFP658 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

TagRFP658

Tags

no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

Plasmid

#217709

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL4 (ADGRL4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-406

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC1 CTF-FLAG

Plasmid

#217684

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2447

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEND-349_Cacnes_ori

Plasmid

#225604

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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myc-KIF5B

Plasmid

#127617

Purpose

N-terminal myc-tagged mouse KIF5B

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5B (Kif5b Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-MYRIP

Plasmid

#89586

Purpose

Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYRIP (Myrip Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-MFA-NFAPoptim

Plasmid

#221090

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pp-mCAT1

Plasmid

#83948

Purpose

This plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectors

Depositor

Vincent Lotteau, Philippe Mangeot

Article

Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

Insert

mCAT1 (Slc7a1 )

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mSTING

Plasmid

#214156

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (Sting1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

Plasmid

#217719

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2522

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

Plasmid

#226515

Purpose

Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Cox8a(4x)-SNAP-tag2-T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pC1 Grx1-roCherry-mito

Plasmid

#141069

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Tags

dMTS

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threoni…

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-eGFP

Plasmid

#116926

Purpose

Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

eGFP

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-DEST47-SIRT1

Plasmid

#242131

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-RPB1

Plasmid

#231722

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

POLR2A (POLR2A Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAIP hIL2 co

Plasmid

#90513

Purpose

Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP

Plasmid

#215055

Purpose

Expresses EGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pC081: pAAV.CMV.mCherry

Plasmid

#205744

Purpose

AAV vector for expressing CMV-driven mCherry reporter gene.

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,580 results for: Ski;