We narrowed to 15,323 results for: pET

Showing: 4851 - 4900 of 15323 results

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T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

Plasmid

#208596

Purpose

Production and purification of catalytically active SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

SynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

Plasmid

#208597

Purpose

Production and purification of catalytically inactive SynCas.v12

Depositor

Omar Akbari

Article

Brogan et al bioRxiv 2024.02.23.581838; doi: https://

Insert

dSynCas.v12

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM257

Plasmid

#226901

Purpose

6xHis-PSP-MBP-Pex3(40-441)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex3 (PEX3 Budding Yeast)

Tags

6xHis-PSP-MBP

Expression

Bacterial

Mutation

amino acids 40-441

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM262

Plasmid

#226906

Purpose

20aa^Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

-PSP-6xHis-FLAG

Expression

Bacterial

Mutation

1-253

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM252

Plasmid

#226896

Purpose

MBP-Pex15(1-327)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-327))

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM245

Plasmid

#226889

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-PANN

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM250

Plasmid

#226894

Purpose

Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-253)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM251

Plasmid

#226895

Purpose

MBP-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-309)

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM247

Plasmid

#226891

Purpose

6xHis-PSP-GS-Hcp1(1-162)-(6aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-Hcp

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM246

Plasmid

#226890

Purpose

6xHis-PSP-GS-ccHex-(9aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission-cchex

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM284

Plasmid

#226122

Purpose

Expression of cdc48 E588Q

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)

Expression

Bacterial

Mutation

E588Q

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM290

Plasmid

#226127

Purpose

Expression of His6-GST-3C-Npl4

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 (HIS6 Budding Yeast)

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM289

Plasmid

#226126

Purpose

Expression of Npl4 D460K/Y461A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 D460K/Y461A (NPL4 Budding Yeast)

Expression

Bacterial

Mutation

D460K/Y461A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
paM291

Plasmid

#226128

Purpose

Expression of His6-GST-3C-Npl4 T255A/T571A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 T255A/T571A (HIS6 Budding Yeast)

Expression

Bacterial

Mutation

T255A/T571A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM288

Plasmid

#226125

Purpose

Expression of Npl4 W252A/R253E

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 W252A/R253E (NPL4 Budding Yeast)

Expression

Bacterial

Mutation

W252A/R253E

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM285

Plasmid

#226123

Purpose

Expression of cdc48 W561A/Y562A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 W561A/Y562A (CDC48 Budding Yeast)

Expression

Bacterial

Mutation

W561A/Y562A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP578

Plasmid

#222957

Purpose

Expresses R144S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 144 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP577

Plasmid

#222956

Purpose

Expresses K142S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Lysine 142 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP576

Plasmid

#222955

Purpose

Expresses R141S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 141 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP575

Plasmid

#222954

Purpose

Expresses R36S LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP573

Plasmid

#222952

Purpose

Expresses E40H LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Glutamate 40 to Histidine

Promoter

T7

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQA-LP572

Plasmid

#222951

Purpose

Expresses E40A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Glutamate 40 to Alanine

Promoter

T7

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pE5771

Plasmid

#170778

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pE5773

Plasmid

#170780

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Article

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

GentR

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sCatch-GFP-6xHIS

Plasmid

#186904

Purpose

Bacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

sfGFP-SpyCatcher003-sfCherry1-10-6xHIS

Tags

6xHis and sfGFP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sTag-BFP-6xHIS

Plasmid

#186905

Purpose

Bacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-6xHIS

Tags

tagBFP, 6xHis

Expression

Bacterial

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWDY1211

Plasmid

#138591

Purpose

Expresses NatL2 in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BBB

Plasmid

#127470

Purpose

coding sequence for synthetic zinc finger ZF-BBB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BBB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BCC

Plasmid

#127484

Purpose

coding sequence for synthetic zinc finger ZF-BCC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCC synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-ACA

Plasmid

#127466

Purpose

coding sequence for synthetic zinc finger ZF-ACA

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ACA synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BCD

Plasmid

#127472

Purpose

coding sequence for synthetic zinc finger ZF-BCD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BAB

Plasmid

#127469

Purpose

coding sequence for synthetic zinc finger ZF-BAB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BAB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BDC

Plasmid

#127473

Purpose

coding sequence for synthetic zinc finger ZF-BDC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDC synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BDD

Plasmid

#127474

Purpose

coding sequence for synthetic zinc finger ZF-BDD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-ADD

Plasmid

#127468

Purpose

coding sequence for synthetic zinc finger ZF-ADD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-ADB

Plasmid

#127467

Purpose

coding sequence for synthetic zinc finger ZF-ADB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-125

Plasmid

#104636

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-413

Plasmid

#104639

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_Myc-Fen_EL5

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pM2P20X

Plasmid

#108210

Purpose

Expresses MSePCNA2-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pS3C

Plasmid

#108211

Purpose

Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA3-CYP101A1 fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pM3C

Plasmid

#108212

Purpose

Expresses MsePCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA3-CYP101A1 fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pM1R

Plasmid

#108208

Purpose

Expresses MsePCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdR fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pS1R

Plasmid

#108207

Purpose

Expresses SsoPCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA1-PdR fusion protein

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri-4a-Strep

Plasmid

#61338

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag, Strep-tag, and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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We narrowed to 15,323 results for: pET