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piRFP720-N1

Plasmid

#45461

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Tags

myc

Expression

Mammalian

Promoter

CMVf

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/TREPittdU3

Plasmid

#18659

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Available Since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV Lex VP16 HA (P#1708)

Plasmid

#14593

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Tags

HA and VP16

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
YFP-CaaX

Plasmid

#155233

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

YFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

YFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-LAC

Plasmid

#64965

Purpose

mini-Tn7 vector with Ptac expression cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET15-UbE2D2

Plasmid

#15783

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-wtVHL

Plasmid

#13390

Depositor

Lucy Anderson

Article

Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

Insert

von Hippel-Lindau (Vhl Rat)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pBSKΔB- CAG-rtTA2sM2-IRES-tTSkid-IRES-Neo

Plasmid

#62346

Purpose

Expresses rtTA2sM2 and tTSkid in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

rtTA2sM2-IRES-tTSkid

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSP64-Vasa-GFP

Plasmid

#135595

Purpose

Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Yajima et al Development. 2015 Jun 1;142(11):1960-70.

Insert

Vasa-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Promoter

SP6

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RFP

Plasmid

#22910

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

Chicken Beta-actin promoter driving DsRedExpress

Use

AAV; Adeno-associated virus

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pTOX3

Plasmid

#127448

Purpose

Encodes Tse2 toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Article

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge