19,624 results

1. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Mutation

   none

   Promoter

   PGK

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-Gal3

   Plasmid

   #85662

   Purpose

   Fluorescent reporter for lysosomal damage

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   LGALS3 (LGALS3 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-inducible Luciferase reporter

   Plasmid

   #64127

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   TetOx13-CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC013 - Twinstrep-SUMO-huLwCas13a

   Plasmid

   #90097

   Purpose

   Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   6xHis-Twin Strep-SUMO

   Expression

   Bacterial

   Available Since

   June 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCX419

   Plasmid

   #229216

   Purpose

   All-in-one plasmid for labeling of XIST RNA via Csm complex

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. NA20 LN16

   Plasmid

   #101870

   Purpose

   Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

   Depositor

   Paul Clapham

   Article

   Peters et al J Virol. 2004 Jul;78(13):6915-26.

   Insert

   Env (env )
   

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pMK345 (Hygro-P2A-mAID-mClover)

   Plasmid

   #121179

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mClover
   

   Use

   CRISPR

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Stephen Elledge, Thomas Westbrook

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYFP-RPB1aAmr

    Plasmid

    #75284

    Purpose

    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

    Depositor

    Xavier Darzacq, Robert Singer

    Article

    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    N792D alpha-amanitin–resistant

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Fos-CreERT2

    Plasmid

    #194643

    Purpose

    Expresses Cre-ERT2 under the control of c-Fos promoter

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    Fos-CreERT2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_PGK_antiCD19_synNotch_TetRVP64

    Plasmid

    #79126

    Purpose

    Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    antiCD19_synNotch_TetRVP64
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid

    #140978

    Purpose

    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaz-Rluc8 (GNAZ Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSGV-1D3-28Z All ITAMs intact

    Plasmid

    #107226

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs intact

    Depositor

    Steven Rosenberg, James Kochenderfer

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z All ITAMs intact
    

    Use

    Retroviral

    Tags

    None

    Mutation

    None

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 HA Arf1

    Plasmid

    #10830

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf1 (ARF1 Bovine)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo-sOVA

    Plasmid

    #25098

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    soluble ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUltra

    Plasmid

    #24129

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

    Depositor

    Malcolm Moore

    Article

    Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-BRD2

    Plasmid

    #65376

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD2 (BRD2 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-GCN5

    Plasmid

    #65386

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    GCN5 (KAT2A Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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