Showing: 81 - 100 of 415 results
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Plasmid#19312DepositorInsertRHEB Ras homolog enriched in brain (RHEB Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Top7 Eb13 triple
Plasmid#12475DepositorInsertTop7 Eb13 triple
UseTags6xHisExpressionBacterialMutationEb13 triple. Top7 extended.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCB150-2_GFP-EB1
Plasmid#46364DepositorInsertEB1 (MAPRE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterpBABEAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-LOV2(fast)
Plasmid#107690PurposeN-terminal half of π-EB1 with fast LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain (I427V, H519L…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-LZ-mCherry-LOV2(wt)
Plasmid#107693PurposeN-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule endsDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Zdk1-EB1C
Plasmid#107695PurposeC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsZdk1 and mCherryExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Zdk1-EB1C
Plasmid#107696PurposeC-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsEGFP and Zdk1ExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorUseTagsExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EB1-EGFP
Plasmid#118084PurposeExpresses EB1-EGFP in mammalian cellsDepositorInserthuman EB1 (MAPRE1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailabilityAcademic Institutions and Nonprofits only -
pEB1-2ndVal mGFPmut2
Plasmid#104033PurposePlasmid encoding 2ndVal mGFPmut2DepositorInsert2ndVal mGFPmut2
UseLow copyTagsExpressionBacterialMutationmGFPmut2 with valine inserted in 2nd positionPromoterproCAvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-RL-ZEB1
Plasmid#35535DepositorInsert3'UTR of ZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mApple-LZ-LOV2
Plasmid#197418PurposeMammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEB1deltaC-2xEGFP shRNA resistant
Plasmid#37925DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationdeletion after aa248, TTCCTGT=>TCCCGGTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag Raptor Rheb15
Plasmid#26634DepositorInsertraptor-Rheb15 (RPTOR Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 415 results