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Showing: 81 - 100 of 114 results

  3. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  4. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51087
    Purpose
    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Availability
    Academic Institutions and Nonprofits only
    51087-plasmid-map-sequence-id-77369

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  9. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Availability
    Academic Institutions and Nonprofits only
    51083-plasmid-map-sequence-id-175189

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  11. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid
    #111394
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    GCaMP6f
    Use
    AAV
    Tags
    6xHis, Myc, T7, and Xpress
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Availability
    Academic Institutions and Nonprofits only
    111394-plasmid-map-sequence-id-212394

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  15. Salsa6f

    Plasmid
    #140188
    Purpose
    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)
    Depositor
    Michael Cahalan
    Article
    Dong et al Elife. 2017 Dec 14;6. pii: 32417. doi: 1
    Insert
    Salsa6f
    Use
    Tags
    tdTomato-v5-GCaMP6f
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Immediate Early
    Availability
    Academic Institutions and Nonprofits only
    140188-plasmid-map-sequence-id-316791

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  16. pHJL2

    Plasmid
    #199377
    Purpose
    Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::SL2::mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199377-plasmid-map-sequence-id-390940

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  17. pSiM2

    Plasmid
    #199381
    Purpose
    Encodes dual cistronic membrane localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199381-plasmid-map-sequence-id-391045

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  18. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199373-plasmid-map-sequence-id-390590

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  20. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Use
    Tags
    His and mRuby3
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

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Showing: 81 - 100 of 114 results