Showing: 21 - 40 of 246 results
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Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI
Plasmid#131479PurposeEndogenous tagging of βactin: N-terminal (amino acid position: D2)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP
Plasmid#129747PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-mEGFP
Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP (ACTB Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-iTagRFP-T
Plasmid#115915Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T (ACTB Human)
UseDonor templateTagsTagRFP-TExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-iTagRFP-T
Plasmid#115916Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T
UseDonor templateTagsTagRFP-TExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-mEGFP
Plasmid#115917Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorInsertgRNA and GFP donor
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailabilityAcademic Institutions and Nonprofits only -
pODN-mR2-ACTB
Plasmid#183868PurposeRepair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.DepositorInsertACTB homology arms with mRuby2-linker (ACTB Human)
UseCRISPR; Donor templateTagsmRuby2-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ACTB_sgRNA
Plasmid#183885PurposepX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pOC1 Halo-Actb KI
Plasmid#183426PurposeCreON knock-in for Halo-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and Halo donor
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOC2 GFP-Actb KI
Plasmid#183429PurposeCreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and GFP donor
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pOC4 Actb-mRuby3 KI
Plasmid#183437PurposeFlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and mRuby3 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-ECFP-Actb
Plasmid#169787PurposeAn actin FRET sensor. Must be expressed with Venus-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsECFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
AICSDP-15:ACTB-mEGFP
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV_Actb NHEJ donor
Plasmid#97316PurposeNHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb NHEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HMEJ donor
Plasmid#97318PurposeHMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb HMEJ donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 246 results