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pmVenus(L68V)-mTurquoise2

Plasmid

#60493

Purpose

Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

SYFP2-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1

Plasmid

#81047

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-CAAX

Plasmid

#75153

Purpose

Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mVenus-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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pKK-mVenus-TEV

Plasmid

#105774

Purpose

Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mVenus-TEV

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mVenus

Plasmid

#105791

Purpose

Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mVenus

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus JBC

Plasmid

#103987

Purpose

Plasmid encoding mVenus JBC

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus JBC

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Availability

Academic Institutions and Nonprofits only

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pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymVenus-CaURA3

Plasmid

#118459

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSW549 YFP/mVenus

Plasmid

#115984

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mVenus

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mVenus-proα2(I)-G610C

Plasmid

#119839

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSHDY-Prha-mVenus_rhaS

Plasmid

#137662

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

Prha:mVenus

Use

Tags

Expression

Bacterial

Mutation

rhaS (Arg218Leu)

Promoter

Prha

Availability

Academic Institutions and Nonprofits only

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pSHDY-PL03-mVenus_tetR

Plasmid

#137663

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the repressor tetR, as well as aTc-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

PL03:mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL03

Availability

Academic Institutions and Nonprofits only

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pSHDY-PvanCC-mVenus_vanR

Plasmid

#137664

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

PvanCC:mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

PvanCC

Availability

Academic Institutions and Nonprofits only

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pSHDY*in_PpetE:BCD2:mVenus

Plasmid

#133970

Purpose

Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

Depositor

Pia Lindberg

Article

Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

Insert

mVenus

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

inserted gene is codon optimized for Synechocyst…

Promoter

PpetE:BCD2

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mVenus

Plasmid

#153202

Purpose

Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the CAG promoter

Depositor

Lynette Lim

Insert

membrane-tagged-Venus

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-mVenus

Plasmid

#153204

Purpose

Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter

Depositor

Lynette Lim

Insert

membrane-tagged-Venus

Use

Retroviral

Tags

Expression

Mutation

Promoter

rat synapsin

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-mVenus-TOSI

Plasmid

#172491

Purpose

mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

Pdcd4 (Pdcd4 Mouse)

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

MLV LTR

Availability

Academic Institutions and Nonprofits only

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cyterm-ymVenus pDRF1-GW

Plasmid

#168079

Purpose

Expresses Cyterm-mVenus to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-mVenus

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CαN15-mVenus

Plasmid

#168486

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-CβN15-mVenus

Plasmid

#168496

Purpose

Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge