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Showing: 1 - 20 of 169 results

  2. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid
    #70666
    Purpose
    Reduced the expression of gamma-tubulin in murine cells
    Depositor
    Maria Alvarado-Kristensson
    Article
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Insert
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    Use
    U6 promoter
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    70666-plasmid-map-sequence-id-133021

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  3. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

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  4. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

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  5. pAAV-U6-racRNA-C

    Plasmid
    #200826
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200826-plasmid-map-sequence-id-396715

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  6. pAAV-U6-racRNA-D

    Plasmid
    #200827
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    200827-plasmid-map-sequence-id-396773

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  13. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  20. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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Showing: 1 - 20 of 169 results