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Showing: 1 - 20 of 76 results

  6. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Neurog2 (Neurog2 Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Insert
    dCas9 and gRNA targeting Actc1 (Actc1 Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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  14. LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,āˆ†318-334)_WPRE_hGHpA

    Plasmid
    #47458
    Purpose
    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (Neurog2)-GS-CIB1(NLS*,āˆ†318-334)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
    47458-plasmid-map-sequence-id-72985

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  16. CBIG-Ngn2

    Plasmid
    #48708
    Purpose
    bicistronic expression of Ngn2 and EGFP
    Depositor
    Jeffrey Macklis
    Insert
    Neurog2 (Neurog2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/beta-actin
    Availability
    Academic Institutions and Nonprofits only
    48708-plasmid-map-sequence-id-70633

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  18. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, Sā€¦
    Promoter
    CAG, EF1a, and TRE3G
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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Showing: 1 - 20 of 76 results