Addgene

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LT3GEPIR

Plasmid

#111177

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK

Plasmid

#133080

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full length

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Avs4_Sulfurospirillum_sp.

Plasmid

#188801

Purpose

Avs4 from Sulfurospirillum sp.

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4

Use

Tags

Expression

Bacterial

Mutation

Promoter

EcAvs4 native promoter

Availability

Academic Institutions and Nonprofits only

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pIRES_EGFP_GA_KCNE1

Plasmid

#173160

Purpose

For mammalian expression of KCNE1 and creating variants

Depositor

Al George

Article

Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

Insert

KCNE1 (KCNE1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIRES2_EGFP_KCNQ1

Plasmid

#173161

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Article

Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

Insert

KCNQ1 (KCNQ1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Research Square PREPRINT. 18 August 2023

Insert

iSeroSnFR_cytosolic

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pASPIre4

Plasmid

#196656

Purpose

Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)

Use

Tags

Expression

Bacterial

Mutation

SpeI site in Bxb1 CDS

Promoter

rhamnose-inducible promoter

Availability

Academic Institutions and Nonprofits only

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PALI1_PIR_pFB1

Plasmid

#191249

Purpose

Expresses PALI1 from insect cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Tags

MBP

Expression

Insect

Mutation

NA

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pIRESpuro2-UBXD1-3x HA

Plasmid

#31836

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

UBXD1 (UBXN6 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pIRESNEO-Scambio-HA

Plasmid

#33354

Depositor

Nora Heisterkamp, John Groffen

Article

Curtis et al Mol Cancer. 2004 Apr 23;3:10.

Insert

Scambio (ARHGEF40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pIRESneo-mTOR-RR/KI

Plasmid

#31074

Depositor

Jie Chen

Article

Park et al J Biol Chem. 2005 Sep 9. 280(36):32009-1

Insert

mTOR (MTOR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S2035T D2357E

Promoter

Availability

Academic Institutions and Nonprofits only
pIRESneo-mTOR-RR

Plasmid

#31075

Depositor

Jie Chen

Article

Park et al J Biol Chem. 2005 Sep 9. 280(36):32009-1

Insert

mTOR (MTOR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S2035T

Promoter

Availability

Academic Institutions and Nonprofits only
pIRESpuro2 AXL

Plasmid

#65627

Purpose

Expresses AXL on cistronic transcript with puro resistance in mammalian cells

Depositor

Aaron Meyer

Article

Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

Insert

Tyrosine-protein kinase receptor UFO (AXL Human)

Use

Tags

Expression

Mammalian

Mutation

Spurious PDPRPHR on C terminus

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF1-V2

Plasmid

#69045

Purpose

expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

splice variant 2

Promoter

IRES

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF3

Plasmid

#69046

Purpose

expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

IRES

Availability

Academic Institutions and Nonprofits only

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pIRES-HMGA1

Plasmid

#13466

Depositor

Edward Whang

Article

Liau et al Cancer Res. 2006 Dec 15. 66(24):11613-22

Insert

HMGA1 (HMGA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

Plasmid

#48795

Purpose

expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Inserts

YWHAE
YWHAZ

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Availability

Academic Institutions and Nonprofits only

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CD23b in pIRES2eGFP

Plasmid

#19136

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

CD23 beta (FCER2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pIRES-Venus

Plasmid

#22665

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

IRES-Venus

Use

Tags

Expression

Mammalian

Mutation

IRES is EMCV IRES

Promoter

Availability

Academic Institutions and Nonprofits only

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pOpen-SapIR

Plasmid

#165537

Purpose

Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

Depositor

FreeGenes Project, Drew Endy, Jennifer Molloy

Insert

SapI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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