Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 41 - 55 of 55 results

  1. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid
    #189871
    Purpose
    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK1 (NEK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    189871-plasmid-map-sequence-id-374462

    Enlarge

  13. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Use
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

    Enlarge

  14. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

    Enlarge

  15. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

    Enlarge

Showing: 41 - 55 of 55 results