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Showing: 1 - 20 of 381 results

  3. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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  7. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  8. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  9. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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  12. Myc-Nix-S35D

    Plasmid
    #197563
    Purpose
    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)
    Depositor
    Joseph Gordon
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Serine 35 changed to aspartic acid
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    197563-plasmid-map-sequence-id-386250

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Showing: 1 - 20 of 381 results