377 results

1. pIVTRup

   Plasmid

   #101362

   Purpose

   Highly efficient in vitro transcription. Empty backbone.

   Depositor

   Ángel Raya

   Insert

   5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
   

   Use

   For cloning a gene for in vitro transcription pur…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDK1as_T2A_Zeo

   Plasmid

   #118596

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Wnt promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NES-mCherry-PH-BTKx2

   Plasmid

   #183654

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GSK3beta/pET29b

   Plasmid

   #16315

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   GSK3beta (gsk3b.L Frog)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

8. Tg(mpeg1:LifeAct-mRuby)

   Plasmid

   #166977

   Purpose

   labels F-actin in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

   Inserts

   mpeg1 (mpeg1.1 Zebrafish)
   LifeAct (actb1 Frog)
   

   Tags

   Lifeact-mRuby and mRuby

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUG34-NES-EGFP-cPHx3

   Plasmid

   #183669

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Human, Frog)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2 xenopus chordin myc

    Plasmid

    #24682

    Depositor

    Edward De Robertis

    Article

    Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

    Insert

    Chordin
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

11. CaM/pIRES2-eGFP

    Plasmid

    #111499

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Xenopus APC-myc

    Plasmid

    #16686

    Depositor

    Barry Gumbiner

    Article

    Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

    Insert

    APC (apc.L Frog)
    

    Use

    Xenopus expression

    Tags

    Myc

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. xenCGN-Myc-His

    Plasmid

    #236505

    Purpose

    For expression of Xenopus CGN in Insect Cells

    Depositor

    Sandra Citi

    Article

    D'Atri et al J Biol Chem. 2002 Aug 2;277(31):27757-64

    Insert

    CGN (cgn Frog)
    

    Tags

    Myc-His

    Expression

    Insect

    Mutation

    WT

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Xl-VSP1

    Plasmid

    #51880

    Purpose

    expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSP64T (DM#111)

    Plasmid

    #15030

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Expression

    Bacterial

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available Since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-pGBD

    Plasmid

    #26735

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    Rac (rac1 Frog)
    

    Use

    Reporter for rac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Rac binding domain of rhotekin

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid

    #74102

    Purpose

    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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