Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 803 results for: mak

Showing: 1 - 50 of 803 results

  1. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TCTTTCCTCATGCCGCCGgcaggattcgggatctTCAGTAACGCTCGTATGA mak TAL3304 & TAL3305 TCAAGCAGCTGGGTGATGgaacctatggcagcgtTCTAATGGGGAAGAGCAA...

  2. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... the heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right...six or seven components — in most cases, that's making two or more plasmids — it may be more efficient...Fragmid doesn’t have the components I want? You can make your own Fragmid components! Ensure your backbone...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    ... cost to make a plasmid? Doench also calculated the cost of consumable reagents used to make a plasmid...after ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (...works for your experiment. Make your work “high-throughput” Instead of making one plasmid at a time, try...Leeson. Have you ever wondered how long it takes to make a plasmid? Or how much time you have to spend cloning...we wrote a post on the time and cost required to make a plasmid. In 2023 (and at closer to 2 million plasmids...updated this post. How much time does it take to make a plasmid? Figure 2: Survey results from a...Flame depositor John Doench calculated the time to make a plasmid. Doench’s lab in the Broad Institute has...

  4. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...should be on their website or the program booklet. Make sure you’re familiar with the policies before posting... tips not covered here, head over to the “How to Make Friends and Meet People at a Scientific Conference...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...needed to perform GCE are essentially those needed to make site-directed mutagenesis, many researchers new ...for the use of GCE. In short, they’re working to make GCE available for all biomedical researchers. If...

  6. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ... events will generate these types of mutations, making it an ‘easy’ editing job to get done. However, ...repair and genome engineering The easiest way to make a knock-in cell line is to utilize the built-in ...observed when inserts are within 10 bps of the break, making the cut off essentially as part of the templated... distance. Homology arms and types of donors To make sure your donor molecule is used as the template...knock-in site. The length of that sequence and the makeup (single vs. double stranded) all depends on the...If your Cas9 cuts in the coding region of a gene, make sure that the PAM edit you introduce is a silent... and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day or two...

  8. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...obstacles.” – Scott Adams, Dilbert cartoonist What makes a good manager? If that is all it takes, then how... aside from a focus on removing obstacles, what makes a good manager? First, it takes an open mind to ... from being included in organizational decision-making. It is imperative that employees are thanked for...a good performer if they are enough of a jerk to make the rest of the team miserable.   A good manager...

  9. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...a good idea. Make contact with other attendees in advance. Join a LinkedIn group (or make one!) or find...is essentially no better place for a scientist to make new relationships than at scientific conferences...you see enough of them).  Before the conference Make sure you have a business card – even students and...will attend. Research the speakers in advance and make a note of those you would like to try and meet. ...members that have moved on to see if they will attend. Make a date or two for beer, coffee or meals with colleagues...memorable meal is a great way to build relationships. Make a list of experimental problems or questions you...poster roll, say hello. Keep your cards handy and make sure your name tag is always visible. Some people...

  11. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...use in your own lab. How to make your own competent cells It’s easy to make your own competent cells. If...need to be cost and time efficient. We do this by making our own competent cells and using a little-known...will work just fine.  A number of protocols for making competent cells are available online. A few examples...Transformation Kit and Buffer Set  from Zymo Research to make competent cells because cells prepared using this...instructions that came with them. If you decided to make your own competent cells using the protocols provided...control (plate your cells without adding plasmid) to make sure you don’t have contamination.  Also conduct...

  14. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...scientists ask me about mentoring. Here they are: What makes a good Mentor? How do I choose an Advisor/PI who... benefits of taking on my own Mentee? Q1. What makes a good science mentor? I started a conversation ...their experience. This led to the topic of “What makes a good Mentor?” Some of the Tweeted comments: “...good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors like Yoda? If...for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by the Mentee...

  15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...studied PIPs. The straightforward nature of BiFC also makes it easier to generate a large number of constructs...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular expression Whether...localization may differ from those of your BiFC construct, making it difficult to interpret your results. Unfortunately...cells. The ease with which BiFC can be visualized makes it amenable to studying PPIs under relatively physiological...

  16. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...individual features that make each tool unique. The CRISPR Software Matchmaker is composed of these features... the end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought...over another. On the other hand some software tools make use of empirically derived and/or prior knowledge... and parameters. This tapestry of ideas is what makes choosing the right tool confusing and it means that...at the glossary section of the CRISPR Software Matchmaker, it provides a list of terms I think are important...going shopping for one. Use the CRISPR Software Matchmaker to select the best tool based on your needs. ... and cherry pick the features that will actually make a difference in our experiments and lives. To do...

  17. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA). (2) The pre-crRNA...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...ability to precisely target CRISPR to a given locus makes it especially amenable to genetic screens. Pooled...targeting in AT-rich genomes. Cpf1's small size also makes it suitable for multiplexing and use in AAV. Scientists...a specific flanking sequence on the target RNA, making it a very flexible system. Other Applications Scientists...have also used the targeting capability of Cas9 to make other types of modifications at specific loci. Activate...

  18. CRISPR Guide

    Type
    Collection
    ...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect... Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. ...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of a handful of bases,...RNA-binding protein to stabilize pegRNAs Twin PE - two PEs make complementary edits to opposite strands Prime editing...the ability to target almost any genomic locus, makes CRISPR an ideal genome engineering system for large-scale...large-scale, forward genetic screening. CRISPR can make highly specific, permanent genetic modifications...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Flp AND Vcre No References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu...Hafner G, Witte M, Guy J, Subhashini N, Fenno LE, Ramakrishnan C, Kim YS, Deisseroth K, Callaway EM, Oberhuber...Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck DA, Deisseroth K, Awatramani...Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, Holmes A, Hájos ...new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao F, Luo MH, Gong... M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis...Burnham N, Cristiano C, Dorrier CE, Tipton GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott...

  20. Genetic Code Expansion

    Type
    Collection
    ...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration...unallocated codon. Therefore many controls must be used to make sure that these conditions are true. Always express...protein with and without the ncAA in the media to make sure that the full length protein is only made when...

  21. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral...

  22. CRISPR Plasmids - Tagging

    Type
    Collection
    ... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ...alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ...

  23. Bacterial Expression Systems

    Type
    Collection
    ...cell, you will need to tag it with something that makes it easy to see under the microscope. The plasmids...Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent...to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions Easy Split...

  24. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...expression systems. Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  25. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

  27. Antibody Guide

    Type
    Collection
    ...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

  28. Serotype Testing AAV

    Type
    Collection
    ...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  29. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  30. AAV Packaged on Request

    Type
    Collection
    ...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

  31. TALEN Guide

    Type
    Collection
    ... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

  32. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ID Plasmid Gene...

  33. Cre-lox system

    Type
    Collection
    ...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...

  34. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...and improving the orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( 188537 )...

  35. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...

  36. Immunology Research Plasmids and Resources

    Type
    Collection
    ... secreted, (semaphorin) 3E KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5 SEMA3F sema domain, immunoglobulin...domain, secreted, (semaphorin) 3F SEMA-IV, SEMA4, SEMAK SEMA3G sema domain, immunoglobulin domain (Ig), ...H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL SLIT1 slit homolog 1 (Drosophila) MEGF4,...

  37. Zhang Lab CRISPR Page

    Type
    Collection
    ...Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...

  38. Plan Your Experiment

    Type
    Collection
    ...setting up CRISPR experiments. We will focus on making single edits using CRISPR/Cas9 in mammalian cells...gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most cases gRNA...

  39. Validated gRNA Sequences

    Type
    Collection
    ... gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the...GGGGCCACTAGGGACAGGAT 72833 cut S. pyogenes 27052166 Kanemaki mmp21 D. rerio CGGAGCTGATCACTGACA 72890 cut S....

  40. Antibodies

    Type
    Collection
    ...transparency and accessibility in research and therefore makes the sequences of these affinity reagents available...

  41. NETRF

    Type
    Collection
    ...NETRF strongly encourages its funded scientists to make their published materials and resources available...

  42. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic modifications, researchers have...

  43. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. The T375G mutation...

  44. Chemogenetics Plasmids

    Type
    Collection
    ...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the ...

  45. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 ...

  46. Mammalian RNAi Tools

    Type
    Collection
    ...Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R....

  47. Recombinases AAV Preps

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  48. AAV Molecular Tools

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

  50. AAV for Neuronal Tracing

    Type
    Collection
    ... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...
Showing: 1 - 50 of 803 results