We narrowed to 472 results for: Sele
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TypeBlog Post...is constantly in flux and is dependent on the selective pressures placed upon it by its environment and...
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Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...cell type you work with (optimization includes selection of transfection buffer and electroporation pulse... -
Antibodies 101: Flow Cytometry
TypeBlog Post... In this example, the lymphocyte population is selected and analyzed further by plotting CD3 versus CD19... -
Typing CRISPR Systems
TypeBlog Post... RNA interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... experimental requirements, it’s a good idea to select an available kit that best fits your needs and ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...accumulation of harmful mutations More, due to selection from more cycles of proliferation Less Cell ... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:3838/BE_... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection... Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans ID Plasmid Gene...Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI...Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions... -
Botman-Teusink Yeast FP Collection
TypeCollection...GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids... and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene...gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...pay for, but one that you are invited to join (selected due to expertise in field). Publicity in major... -
Viral Vector Guides and Plasmids
TypeCollection...Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors...'s AAV plasmid collection. Addgene also has a selection of in-stock viral preps available. Browse Guides...useful for clinical applications as vaccines. Selecting a Viral Vector Type Each viral vector type exhibits... -
Fluorescent Protein Guide: Biosensors
TypeCollection... We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many more...sugar-binding protein into a FRET sensor with high selectivity for sucrose. J Biol Chem. 2006 Oct 13. 281(41..., NTS, UCN, SST, and CRF A tool kit of highly selective and sensitive genetically encoded neuropeptide...Constitutive or Cre-dependent) Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning...Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. Mol Cell. 2019 Apr 18;74(2):347-362... -
CRISPR Guide
TypeCollection... complex functions, including the ability to selectively activate/repress target genes, purify specific...additional Cas proteins and PAM sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9...There are many online tools available to help you select an optimized gRNA for your application (see: Plan...is generally low (< 10% of modified alleles). Selecting a target cut site as close as possible to the ...design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity and low ... available in two formats: as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...first, the Liu lab used phage-assisted evolution selection systems to generate ABE8e(TadA-8e V106W), which... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection.... Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers were selected from the genome.... Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell... -
Plasmids for Stem Cell Research
TypeCollection...table by delivery method, species, or PI and then select the article link to find more information. Delivery... type, delivery system, species, or PI and then select the article link to find more information. Convert... Fibroblasts Sensory Neurons Lentiviral Mouse Selective conversion of fibroblasts into peripheral sensory... -
Using AAV for Neuronal Tracing
TypeBlog Post...Schwab, M., Suda, K., and Thoenen, H. (1979). Selective retrograde transsynaptic transfer of a protein... -
CRISPR Plasmids - Epigenetics
TypeCollection... Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI ...