...EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast growth... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

...Addgene's TALEN Guide. These collections and accompanying documentation allow one to efficiently assemble...Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer and tetramer...TALEN Kit All possible tetramers (264) of RVDs, compatible with Golden Gate TALEN and TAL Effector Kit 2.0...i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden Gate TALEN kit, (ii) a lacZ ...

... Guide for more information about: Lentiviral components 2nd versus 3rd generation lentiviral systems ...feature of lentivirus used in research is that the components necessary to produce an infectious viral particle...called packaging and envelope plasmids) encode components of the viral capsid and envelope and are used...transfer plasmids along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making...

...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...to identify antibody escape mutants and for comprehensive mapping. Some of the following plasmids are ... Library of Spike protein RBD mutations for comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor... of variant Spike protein RBD mutations for comprehensive mapping (Jesse Bloom, Tyler Starr). External...

...CRISPResso CRISPResso is a robust and easy-to-use computational pipeline that enables accurate quantification...visualization of CRISPR-Cas9 outcomes, as well as comprehensive evaluation of their effect on the coding sequence...sequence (without PAM sequence) can be provided, to compare the predicted cleavage position to the position...sequencing, WGS datasets and allows the direct comparison of individual experiments. PubMed PMID 27404874...

...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Plasmids MORF Pooled Library The MORF library is a comprehensive human transcription factor collection that contains...Pooled Library The MORF pooled library is a comprehensive collection of human transcription factor open...

...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... turned dCas9 into a customizable DNA labeler compatible with fluorescence microscopy in living cells....

... research in small biotech and pharmaceutical companies. The RESOLUTE project has received funding from...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow...efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry...

...The siRNA then binds the RNA-induced silencing complex (RISC). RISC unwinds the double-stranded siRNA ...available. To find plasmids containing other RNAi components, like argonaute, please search our full site ...., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...

...below and not included in this table. For a more complete list of plasmids that can be used in worms, search...elegans. WormBase - A consortium of biologists and computer scientists dedicated to providing the research...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed...

...Density centrifugation purification Addgene’s comprehensive quality control process: Titering Purity assessment... amplify DNA, produce the prep, and perform comprehensive quality control processes to make sure it is...researchers with troubleshooting and results that complement published data, capturing the insights that often...

... - a public web resource composed by a collection of databases, computational and experimental resources...monocots and dicots - Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency...

...for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable Target Gene...

...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol...

...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to clone the... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

...designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant ...Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting...

...knockout, repress, and activate gene targets. To accomplish this they have taken advantage of the Zhang lab...guide within a U6-guide cassette. This process is compatible with all pX458 family plasmids. Additional Cloning...

... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...

...γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and several structural and enzymatic...gets integrated into the host's genome by the accompanying integrase protein. When scientists discuss retrovirus...

...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency of editing...additional sgRNA PE4 - PE2 plus MLH1 (a protein component of MMR) mutant to inhibit MMR PE5 - PE3 plus MLH1...

...different experimental goals. How does this method compare to other similar methods? Where does this method...For All Attendees: To help us best prepare and compile resources for this meeting's publication, we are...

...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia...

...). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component involved...

... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate...

...to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian... Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest fused between...

...and related MECP2 disorders. The RSRT has a comprehensive, strategic and aggressive plan to use gene editing...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...

...using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus... particular organism, and the library is only compatible with cells derived from that organism. All of...

...Chris Garcia 157115 pD649-HAsp-TREM2-COMP5AP-AviTag-9xHis TREM2 COMP5AP, Avi, His CMV, SP6 Alzheimer's Chris...Chris Garcia 157447 pD649-HAsp-CSF1R-COMP5AP-AviTag-9xHis CSF1R COMP5AP, Avi, His CMV, SP6 Hereditary diffuse...Chris Garcia 157495 pD649-HAsp-ERBB4-COMP5AP-AviTag-9xHis ERBB4 COMP5AP, Avi, His CMV, SP6 ALS Chris Garcia...

...MultiSite Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with... and plasmids that provide Cas9 variants. All components are then assembled into a single T-DNA binary...

...biology plasmids related to metabolic pathways and components. Examples Include: Biofuels Natural Product Pathways...

...scientists can design de novo systems using modular components or take advantage of existing GFP-lines for cell-specific...

... will use state-of-the-art experimental and computational approaches to model, predict, characterize, ...

...Florida State University has contributed their comprehensive collection of ORFs tagged with a variety of ...

... targeted genome editing. RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses...

...and treatment. On each pathway page, click on a component to find plasmids with your gene of interest. Browse...

...affinity purification against the epitope tag. Compared to conventional methods for genomic purification...

...Davidson FP Collection Browse the Michael Davidson comprehensive collection consisting of ORFs tagged with a ...