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We narrowed to 1,100 results for: Ide

Showing: 41 - 80 of 1100 results

  2. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA...for use in zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome... gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type ...RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’...web-based ZiFiT Targeter software to enable identification of potential RGN target sites and to facilitate...facilitate design of short oligonucleotides needed to construct gRNA expression vectors. More information ...

  3. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning...to faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  4. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Vector Type Promoter PI Publication...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  5. Optogenetics Plasmid Collection

    Type
    Collection
    ...ready-to-use AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter..."Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter...Optogenetics Guide AAV Viral Service Biosensors Chemogenetics Plasmids Chemogenetics Guide Fluorescent ...Fluorescent Protein Guide Microbial Opsin Plasmids Optical Switch Plasmids Background Optogenetics plasmids encode...opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas...Variants of channelrhodopsin have been engineered or identified to increase the spectral and kinetic properties...in these tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research...

  6. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...bacteria. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs is not considered "scarless...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  7. Bacterial Expression Systems

    Type
    Collection
    ...structure, and interactions! Plasmid ID Promoter Epitope Tag(s) ID Purpose pDEST-HisMBP 11085 Tac (lactose...harness bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini...of interest does when expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones...systems with your gene/protein of interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids...activate expression of the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various Plasmids...localization to aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids with a wide variety of... like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. coli strains for protein expression...

  8. Resolute Plasmid Collection

    Type
    Collection
    ...efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry...target the solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561...transporters in the human genome with approximately 450 identified members. Many SLCs have mutations associated ...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...

  9. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression... the TEF1 or PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication...

  10. COVID-19 Resources

    Type
    Collection
    ...plasmids available to industry is continually growing. ID Plasmid Description Industry PI Return to top Other...Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control...SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI For more information...helpful for COVID-19 research in the table below: ID Plasmid Description Industry PI Do you have a suggestion...Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive ... or Inserts Several mammalian genes have been identified as having a key role in coronavirus infection...and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools SHERLOCK and...

  11. Distribution to Industry

    Type
    Collection
    ...that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description...as well as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications...intellectual property rights, and provides liability protection for material providers. Addgene’s deposit agreements...Kit for using comprehensive end-joining ligation fidelity and bias data to predict high accuracy junction...

  12. Retrovirus Plasmids

    Type
    Collection
    ...Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging ...for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA...Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors...insertional mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging...

  13. Worm Expression Resources

    Type
    Collection
    ...used in worms, search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing...C. elegans, C. briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed...Caenorhabditis elegans ( C. elegans or “the worm”) is widely used as a genetic model organism due to their rapid...community that aren't in the repository? Help us widen our resources for the community by depositing your...ordered individually. CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology....elegans. Silencing Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...such as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return...as the promoter or fluorescent protein of choice. ID Plasmid Description Promoter PI Return to top Other...like... Plant CRISPR resources Fluorescent Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription.... The tables below highlight plasmids spanning a wide variety of molecular biology technologies: from ...Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...

  15. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP...May Also Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization...Institute for Cell Science has several protocol videos available that may be helpful for working with ...

  16. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP...will instead form white colonies. Adenoviral Vector ID Purpose pAdx-CMV-LacZ 73345 Expresses lacZ from the...Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources Addgene...Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors Kazuhiro... of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for packaging...

  17. Tags and Other Markers

    Type
    Collection
    ... antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species...

  18. Neuroscience

    Type
    Collection
    ... antibodies and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species...

  20. TREAT-AD Plasmid Collection

    Type
    Collection
    ...table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...

  21. Arf GTPase Family

    Type
    Collection
    ...plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1...plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1... is regulated both upstream (by GEFs, guanine nucleotide exchange factors), and downstream (by GAPs, GTPase...

  22. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ... other cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link...Initiative (iNDI) Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research...project aims to generate and characterize single nucleotide variant lines in an isogenic cellular background...

  23. KLF Research Plasmids

    Type
    Collection
    ...Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

  26. TALEN Expression Vectors

    Type
    Collection
    ... domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70...methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details...

  27. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...packaged in AAV particles. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  28. Brain Armamentarium

    Type
    Collection
    ...Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo...

  29. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1...be helpful to the scientific community. Penn ID Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-...Controls To look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...Penn Vector Core) and Addgene have partnered to provide high-quality AAV vectors to the academic research...

  31. Lentivirus Plasmids

    Type
    Collection
    ...with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253 pRSV-Rev...CRISPR Empty gRNA plasmids table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro...Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these...increased researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components...infectious viral particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically...transfer plasmids," which can be modified to encode a wide range of gene products. The main restrictions on...

  32. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...target sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  33. CRISPR Plasmids - Parasites

    Type
    Collection
    ... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication... gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  35. All Antibodies

    Type
    Collection
    ... Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype...based on our in-house testing and data provided by trusted outside labs – with full experimental details...accessibility in research. For each antibody, we provide important details including the protein and plasmid...about antibodies and their use, visit our Antibody Guide . Browse or search the table below or visit the ...you choose, store, or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody...

  36. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...was originally used. Here are a few questions to consider when choosing a gRNA: Does a given gRNA sequence...the deposit process. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna...

  37. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality ...and Customer Support Teams are also available to provide assistance at [email protected] . Don’t See What...

  38. TALEN Engineering

    Type
    Collection
    ...target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers ...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced... a new window) is available to enable users to identify both dimeric TALEN and monomeric TALE transcriptional...additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual...

  39. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core catalog items. Virus-associated... experimental conditions. Protocols Written and video protocols for how to use your viral prep and how... Addgene, with details about storage. Biosafety Guide Documentation for your institutional biosafety office...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...used to make the virus. In order for Addgene to provide viral vector services to you, we must ensure that...

  40. Luciferase Plasmid Collection

    Type
    Collection
    ...plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc...plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter Description PI 60226...that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top ...emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching ...depending on the application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more...systems. Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase...level of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many...
Showing: 41 - 80 of 1100 results