.... Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one inactivated one... available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while... SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes NNTA, a PAM sequence... create the adenine base editor. The TadA domain has also been further evolved to catalyze cytidine deamination...editing requires more optimization and typically has lower efficiency (< 50%) than base editing (> 90%...modifications to eliminate target gene function. It has also been used extensively to screen for novel genes... those for yeast or drosophila . Although CRISPR has been less widely used in bacteria due to technical...

...Technological Advances CRISPR technology development has occurred at a truly astonishing pace, with work directed...Directions It’s amazing to see how much progress has been made in just five years, with the skyrocketing...see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the CRISPR... find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian...

...research topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants...

... immune response to it. Once the immune response has developed, the antibodies are collected, tested against...epitopes on constant regions of other antibodies. This has useful research applications, and is covered in the...exist as monomers. Each antibody class therefore has a valency, or number of binding sites available per...of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description Configuration... must be able to detect if and where an antibody has bound. Signaling molecules , proteins that emit a...to avoid confusing data. Check that your machine has appropriate lasers and filters for each fluorophore...

...plasmid DNA for use with virus-mediated delivery has many advantages for research. Viruses facilitate ...Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration...

...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

... Shannon Boye’s lab. It is derived from AAV2 and has the following mutations: Y444F, Y500F, Y730F, T491V... lab. AAV44.9(E531D) The AAV44.9(E531D) serotype has increased transduction and lateral spread in the ...

...numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...

... Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their web-based ZiFiT Targeter software...

...these tools in well-defined cellular sub-populations has generally been limited to single-component cellular...Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable...using EYFP and ChR2-EYFP as payloads). This approach has been broadly applied using multiple recombinase-expression...

...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer signaling...

...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...

...parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some of these parasites...

...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...

...Recommended Applications Browse Epitope Tag Antibodies IPI has developed recombinant versions of antibodies that...

...Vectors Communities Find a scientific community that has established a common place for deposited plasmids...

...animals. Further development of the original iRFP has resulted in a split fluorescence complementation ...

... Neurodegeneration Research Collection Addgene has assembled the following to help you find information...Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new window...

... your gene of interest once its appropriate cDNA has been fused to the MS2 binding sites within this plasmid...expression only at a specific time. This collection has a variety of plasmids whose expression levels have...

...to top AAV - In vivo Genome Editing The Zhang Lab has both single- and dual-vector systems that allow the...uses a smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and packages both the... : PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA...