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We narrowed to 1,006 results for: RING

Showing: 81 - 120 of 1006 results

  1. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function ...used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually...of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...Joung et al., PNAS 2000 ). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs ...For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

  2. Synthetic Biology - Overview

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson...Weiss Synthetic biology describes the rational engineering of biological systems for novel functions. Addgene... biologists around the world to facilitate the sharing of parts and information. Addgene can help you ...Regulation Browse plasmids containing both naturally occurring and synthetic regulatory elements, including promoters...Bio-Energy Institute Synberc - Synthetic Biology Engineering Research Center The PLOS Synthetic Biology Collection...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive, facile, and efficient...site. Design additional primers to analyze for scarring (small indels produced at the sgRNA cleavage site...to amplify the sgRNA target site to examine for scarring. This may be useful to characterize the non-deleted...Minimize the time between electroporation and transferring the solution into media to enhance cell viability...of Transfected Cells Prepare cells for FACS by filtering them through a 50 μm filter into a FACS tube. ...manuscript here: Bauer et al, 2014 322.2 KB Genome engineering using the CRISPR-Cas9 system. Ran FA...

  4. Antibody Guide

    Type
    Collection
    ...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...valency of 2 IgG1; IgG2; IgG3; IgG4 IgM Produced during the primary immune response; found mostly in blood...protein:protein complex. Due to their increased manufacturing requirements, monoclonal antibodies are typically...tolerate freeze-thaw cycles. Here are a few tips for storing your antibody: Antibodies conjugated to fluorophores...a similar assay type) and search again. After filtering, narrow down further by company, price, and other...assay with the results of a non-antibody assay measuring either the same output or a proxy for that output... antibody validation This method validates by comparing your antibody of interest to a second, independent...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ...DMA, HLADM, RING6 HLA-DMB major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major...a pathogen and is the dominant defense strategy during the first several days following exposure to a ...communicate with each other across long distances. During infection and inflammation, the different cytokines...create pores within an infected cell membrane, triggering cell death. TCR Signaling The T cell receptor...macrophages and sense conserved microbial molecules during an infection. When TLRs sense a microbial pathogen...peptidase 7) D6S216, D6S216E, LMP7, MGC1491, PSMB5i, RING10, beta5i PSMC1 proteasome (prosome, macropain) 26S...ABCB2, APT1, D6S114E, FLJ26666, FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...separate diseases, will help speed progress towards curing and treating them. Scientists are studying protein... field will advance more quickly with the avid sharing of data, tools, resources, methods, and results...mid-sixties, or late-onset, where symptoms appear during or after a person's mid-sixties. The early-onset...2014 Apr 17. Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Sun et al. Nat... an aggressively funded research agenda and to ensuring the development of improved therapies for those...

  7. AAV for Neuronal Tracing

    Type
    Collection
    ...tracing virus that lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the...starter" cell, but new virus cannot spread to any neighboring cells. To enable monosynaptic spread of this ...newly formed viruses are unable to infect any neighboring cells. Thus, through complementation, G-deleted...released from the starter cell and free to infect neighboring cells via G-protein receptors, which are naturally...

  8. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish...zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') Left half-... publication. References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly...301. PubMed PMID 18657511 . Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

  9. Plasmids for Stem Cell Research

    Type
    Collection
    ...differentiate into many different cell types in the body during development. Unlike other cell types, each stem...and Klf4) that are known to maintain pluripotency during embryonic development. This “reprogramming” of ...0214. Cheng RNA Human Reprogramming factors for delivering synthetic mRNAs encoding human Klf4, Oct4, Sox2...plasmids Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell...separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. ...

  10. Worm Expression Resources

    Type
    Collection
    ...Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring behavioral and...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids...collection of original, peer-reviewed chapters covering topics related to the biology of C. elegans and...

  11. Plant Plasmids and Resources

    Type
    Collection
    ... molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. ...modification of monocot and dicot genomes using genome engineering; validated in tomato, tobacco, M. truncatula ...for chloroplast transformation and metabolic engineering in plants (including tobacco and potato) - Scott...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide...Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL...Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated...

  13. COVID-19 Resources

    Type
    Collection
    ...fluorescent reporter plasmids that have been used for measuring viral entry and activity in neutralization assays...Industry column below. For more information on ordering from a for-profit entity, please see our Plasmid...access to articles related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource...Collection Science Journals Coronavirus Collection Springer Nature SARS-CoV-2 and COVID-19 Articles Wiley ...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR... the CRISPR-Cas9 system can be implemented by delivering Cre and sgRNA to a Cre-dependent mouse or sgRNA... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...

  15. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

  16. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas systems, are programmable endonucleases...Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional pages describing...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Kalle Gehring 45970 pGEX-parkin 77-465 PRKN GST tac Parkinson's Kalle Gehring 45971 pGEX-parkin...Parkinson's Kalle Gehring 45972 pGEX-parkin 219-465 PRKN GST tac Parkinson's Kalle Gehring 45973 pGEX-parkin...Parkinson's Kalle Gehring 45974 pGEX-parkin W403A PRKN GST tac Parkinson's Kalle Gehring 45975 pGEX-parkin...Parkinson's Kalle Gehring 45976 pGEX-parkin C431S PRKN GST tac Parkinson's Kalle Gehring 45977 pGEX-parkin...pGEX-parkin W403A-C431S PRKN GST tac Parkinson's Kalle Gehring 46904 pRK5-EGFP-Tau MAPT GFP CMV Parkinson's, FTD...eIF4G delN83 EIF4G1 V5, MS2 CMV Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary... 86-130 Parkin) PRKN GST tac Parkinson's Kalle Gehring 102361 pcDNA3.1-hSNCA-NE SNCA NE CMV Parkinson'...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components have ... improve the efficiency of editing, including exploring new sources for the reverse transcriptase. Many...

  19. Viral Vectors

    Type
    Collection
    ...viruses with modified viral genomes. Naturally occurring viruses harbor genetic material (termed the viral...alternate (non-virus-producing) genomes, naturally occurring viral genomes have been adapted into a plasmid-based...ability to propagate in the same way that naturally occurring viruses do. Instead, the genes that are required...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis...backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including...zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R package for analyzing ...

  21. Rett Syndrome

    Type
    Collection
    ...regardless of age or symptom severity, suggesting that restoring MECP2 expression in humans could be curative. ...and repository, and CRO for centralized behavior scoring (Link opens in a new window) MMRRC Repository -...opens in a new window) Protocols.io - protocol sharing site Funding Opportunities (Link opens in a new...

  22. Plan Your Experiment

    Type
    Collection
    ...sites are called off-targets and should be examined during gRNA design. In general, off-target sites are not...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...

  23. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... final product should be translated as a single string of amino acids that preserves the sequence...dedicated Cre-lox Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN ... custom TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection...

  24. Bacterial Expression Systems

    Type
    Collection
    ... Systems Bacterial Expression Systems Genome Engineering Visualization and Tagging Purification Controlled... expression systems to find out more! Genome Engineering Although overexpressing or knocking down a gene...different ways to alter bacterial genomes. From recombineering to CRISPR , this collection contains a variety...

  25. Luciferase Plasmid Collection

    Type
    Collection
    ...gene.. TREAT (3( T hree)′- R NA E nd A ccumulation during T urnover) : Single-molecule mRNA turnover biosensor...Lentiviral expression of firefly luciferase Brent Stringer 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral... Insect expression of firefly luciferase Kevin Maringer 131860 pZmUBQ:LUC Firefly CaMV Zea mays expression...

  26. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks &...Regulation. The collection includes both naturally ocurring and synthetic regulatory elements, pre-assembled...

  27. TALEN Expression Vectors

    Type
    Collection
    ... TALengineering Reagents TALEN Expression Vectors TALEN Expression... FLASH assembly methods can be found on the TALengineering website . All three of these methods yield ...

  28. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...researchers at Stanford University’s Department of Bioengineering, to build DNA that will enable researchers ...industrial organizations. For more information on ordering from a for-profit entity, please see our Plasmid...

  29. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...targeted to a site with a spacer of 7 bps and harboring a “+” heterodimeric FokI domain ( Miller et al...targeted to a site with a spacer of 7 bps and harboring a “-” heterodimeric FokI domain ( Miller et al...

  30. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Save time by ordering cloning grade DNA for over 200 of our most popular plasmids!...Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids...

  31. Resolute Plasmid Collection

    Type
    Collection
    .... The combination of metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties...

  32. Mammalian RNAi Tools

    Type
    Collection
    ...as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via transfection or ..., L. (2023). A comprehensive review of small interfering RNAs (siRNAs): mechanism, therapeutic targets...

  33. CRISPR Plasmids and Resources

    Type
    Collection
    ...immune system to a revolutionary tool for genome engineering. CRISPR Topic Page : Want the latest news on ...experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas enzymes generate double strand...

  34. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...of shipment. If any discrepancies are identified during this analysis, you will be notified by email. If...by email before, or within 10 business days of ordering. We will give you the option to proceed with your...

  35. Retrovirus Plasmids

    Type
    Collection
    ...scientists discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally...

  36. AAVED

    Type
    Collection
    ...Ben Deverman Broad Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research ...Deverman, Broad Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan...

  37. Open Enzyme Collection

    Type
    Collection
    ...Researchers can use the collection as the basis of manufacturing their own enzymes by cloning them into an expression...functionality or add new functions through genetic engineering and directed evolution. More information about...

  38. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...site flanked by 15 amino acids on either side occurring within the parent protein. Using the recoded strain... described in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial ...

  39. Deisseroth INTRSECT Collection

    Type
    Collection
    ...animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure 2: INTRSECT...recombinases are present, the introns are excised during mRNA processing, producing a functional molecular...

  40. Retrograde AAV viral preps

    Type
    Collection
    ...vectors undergo quality control, including AAV titering by SYBR green qPCR , confirmation of the transfer...requested criteria. Please consider using fewer filtering options. Browse all Retrograde AAV *The AAV retro...
Showing: 81 - 120 of 1006 results