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... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...powerful tools to control gene expression and study biological targets. The tetracycline (tet) system is one...improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal... to a reverse Tet repressor, or rTetR, which specifically binds the TRE in the presence of tetracycline...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter...

...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...systems and delivery methods . Validate Genetic Modification Once you have successfully delivered the gRNA...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...the edit need to be homozygous? What type of modification is it? Are you modifying the genomic sequence...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to...Drosophila Plant C. elegans Yeast Resources Catalytically dead dCas9 fused to a transcriptional activator...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a ...

...synthetic biology by providing access to new chemical and physical properties for studying, manipulating, and...incorporation of non-canonical amino acids (ncAA) in a protein of interest. Applications By making small changes...acids beyond the 20 standard ones, known as non-canonical amino acids (ncAAs). This strategy, called genetic...a ncAA into a protein of interest, four key modifications to the standard translation machinery are required...reassigned codon. An aaRS enzyme is engineered to specifically load the new tRNA with the ncAA. Together, the...with a different amino acid. To achieve these modifications, two plasmids are generally used. Once these...that contain genes with modified codons for non-canonical amino acid incorporation. ID Plasmid Gene/Insert...

...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...components can be used separately for additional applications. Park & Sheridan et al. (2025) use plasmids ...circuit mapping and reconstruction. Potential applications could also include using individual or subsets...well as barcoding with specific enhancers for morphological readout. How to Use PRISM Barcodes are delivered...AAVs either directly to a region of interest or systemically using a capsid capable of crossing the blood-brain...microscopy workflows to achieve high-resolution optical imaging down to individual synapses. Figure 2: ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . Read...

...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

...synthetic chemical ligands, which have been developed for special functions that vary from purification, visualization...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...levels in lysates or live-cells, thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Karl...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Karl...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Jonathan...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Jonathan... Loren Looger 113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_...sensor Yulong Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor...

... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...- sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

...repository contains many plasmid tools that were specifically designed for use in plants. These resources ...Infrastructure Kit Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains...multi-purpose toolkit that enables targeted, specific modification of monocot and dicot genomes using TALENs and...CRISPR. Successfully validated in tomato, tobacco, Medicago truncatula , wheat, and barley. Return to top ...localization, protein–protein interactions, and other physiological processes in plants. ID Plasmid Description ...an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens in a new window...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Selectable Marker PI Publication Return to...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR ...

... PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Description Gene...Gene/Insert Promoter Selectable Marker PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of...

...antibodies. These monoclonal antibodies undergo application-specific validation and quality control by Addgene...antigen. Addgene supplies a list of recommended applications based on our in-house testing and data provided...with full experimental details in the Antibody Applications section of our product pages. We also include... an antibody’s use is not recommended for an application or species. We currently assess western blot,...scientists to further develop and refine these application lists. The plasmids we use to produce antibodies...Source Species Isotype Reactivity Recommended Applications PI Return to Top Do you have suggestions for...

...) domains that respond specifically to small molecules termed Pharmacologically Selective Effector Molecules...Plasmid Collection Chemogenetics plasmids encode genetically engineered receptors that are activated by specific... to artificially activate or inhibit specific biological processes. Addgene's collection contains two ...Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs...have been engineered to respond to specific physiologically inert small molecules. There are four types...

...subject to Addgene’s Terms of Purchase and the applicable material transfer agreements (MTAs) and limited...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated...be required by your country for the import of biological research materials. Please be aware that additional...contact you directly regarding these charges, if applicable....

... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Genome Engineering...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato. 8 Massimo Scanziani 34910...constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP...

...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...the international research community. The Latin American Zebrafish Network (LAZEN) - LAZEN aims to increase...advanced training of young scientists in Latin America. The Zebrafish Book - A guide for the laboratory...

...Brain Science AAV Enhancer Collection Plasmids Publications Resources Enhancer AAV plasmids are plasmids...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...= Substantial non-specific labeling observed Publications Ben-Simon, Y., Hooper, M., Narayan, S., Daigle...and transgenic mouse lines for genetic access to cortical cell types. Cell , 188 (11), 3045–3064. https:...

...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...

...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our...

...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...

...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins and to find plasmids...Microscopy Resource Center (Link opens in a new window) Leica Microsystems Resources for Microscopy in Life Sciences...

...optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and manipulate...Optogenetics Plasmid Collection Microbial Opsin Plasmids Optical Switch Plasmids Additional Resources Optogenetics...proteins. These fusions can then be used as an optical switch to control protein interactions, dimerization...response spectra) of different types of opsins and optical switches available in these tables, refer to Addgene's... Eligible for Viral Packaging PI Return to top Optical Switch Plasmids Browse, sort, or search the table...

...unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically along the way. Cas3...Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository...biology describes the rational engineering of biological systems for novel functions. Addgene is working...Browse plasmids related to cloning and genomic modification, including shuttle, integration, reporter, and...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...Engineered to use the substrate Akalumine with significantly brighter signal and emission in the near-infrared...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Ppp1r9a Mouse Mouse IgG2a 211585 Anti-Versican [N351/24R] Versican Rat Mouse IgG2a 211587 Anti-GluN3A/NR3A...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...extensively validated for neuroscience research applications. A substantial number of these have been cloned...

... a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter ...Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker...Marker PI Publication Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable...

...K-RAS, N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion...repurposing of open access immunological assay data for translational and clinical research. Sci Data. Feb...and adaptive immune signaling and cell-cell communication. Plasmid...specific to any particular pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial...molecules that enable cells of the immune system to communicate with each other across long distances. During...with virus and, like other cytokines, enable communication between cells of the immune system. Interleukins...are secreted by immune cells and coordinate communication between immune cells over the course of an immune...

...into a genomic attP site. So far, their practical application has been limited by low integration efficiency...other recombinase systems. Enzyme Target Site Canonical Sequence Structure (Length) Common Variants Cre...repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp FRT GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre...

... Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top...Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to...

...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...

...cited in publications. All pricing includes DNA amplification, viral vector production, purification, and ...Additional Resources Addgene's Data Hub Curated technical reports from the research community demonstrating...

...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...