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We narrowed to 808 results for: Eng

Showing: 101 - 150 of 808 results
  1. Viral Vector Guides and Plasmids

    Type
    Collection
    ...produce more virions. B) Viral plasmids have been engineered such that they can be delivered to packaging ...and tissues. Viral plasmids have been carefully engineered for safety and are replication-incompetent. Specific...
  2. Retrovirus Plasmids

    Type
    Collection
    ...types. Wild-type gamma-retroviruses have been engineered into gamma-retroviral vectors for safe use in...Retroviral construct for miRNA expression. Chang-Zheng Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic...
  3. Microbiology Resources

    Type
    Collection
    ...gondii Trypanosoma sp. Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles...Read our CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making...
  4. Promega Plasmid Collection

    Type
    Collection
    ...in real time using full-length proteins or fragments. NanoBRET target engagement (TE) assays (Link opens...
  5. Zebrafish Plasmid Collection

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    Collection
    ...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific...
  6. Open Enzyme Collection

    Type
    Collection
    ...functionality or add new functions through genetic engineering and directed evolution. More information about...polymerase 165543 pOpen-Bstpol Bst DNA Polymerase, Full Length 165544 pOpen-T5gene122 T5 DNA Polymerase 165553...
  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine to tRNA^Sep with anticodon, CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC... U., Rovner, A. J., Aerni, H. R., Rogulina, S., Cheng, L., Olds, W., Fischer, J. T., Söll, D., Isaacs,...
  8. CRISPR References and Information

    Type
    Collection
    ... Manuel Leonetti Lab . For more information, see Peng et al. (2024) (Link opens in a new window) . gRNA...new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids...
  9. Plasmids for Stem Cell Research

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    Collection
    ...Cells Transl Med. 2015 Mar 5. pii: sctm.2014-0214. Cheng RNA Human Reprogramming factors for delivering synthetic...expression signatures. Cell Res. 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon...
  10. Tetracycline Inducible Expression

    Type
    Collection
    ... control gene expression, the system has been engineered with a variety of improvements. A synthetic Tet...systems (Loew et al., 2010; Heinz et al., 2011), engineered to eliminate binding of endogenous mammalian ...
  11. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...fluorescent protein that has a different emission wavelength. For example, if the marker protein is fused ...Patricia Wadsworth 27123 eTC GFP beta-actin full length Actin filaments beta-Actin EGFP Robert Singer 40908...
  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Photoactivatable (Off to On) Excitation and Emission wavelengths after activation. Protein Excitation (nm) Emission...to top Self-Labeling Tags These tags have been engineered to covalently bind a synthetic substrate conjugated...
  13. Synthetic Biology - Algal

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  14. Synthetic Biology - Bacterial

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  15. Synthetic Biology - Fungal

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  16. Synthetic Biology - Mammalian

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  17. Synthetic Biology - Plant

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  18. Synthetic Biology - Worm

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  19. Synthetic Biology - Yeast

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology...
  20. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks &...
  21. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks &...
  22. Depositor Collections

    Type
    Collection
    ...Plasmid Collection E11 Bio PRISM Collection New England BioLabs Cell-Imaging Plasmid Collection Promega...
  23. MAPK Plasmids

    Type
    Collection
    ...You may also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated...
  24. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...
  25. Optogenetics Plasmid Collection

    Type
    Collection
    ... cell. Variants of channelrhodopsin have been engineered or identified to increase the spectral and kinetic...
  26. Mammalian RNAi Tools

    Type
    Collection
    ...Zhang, J., Chen, B., Gan, C., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive review of small interfering...
  27. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...strand). Likewise, adenine base editors have been engineered to convert adenosine to inosine, which is treated...
  28. AAV for Neuronal Tracing

    Type
    Collection
    ...the rabies virus with EnvA, only neuronal cells engineered to express TVA will be infected. Schematic depicting...
  29. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...leukemia virus (M-MLV) reverse transcriptase. Engineering of the PE enzyme and related components have ...
  30. Distribution to Industry

    Type
    Collection
    ... Davidson collection Protein Labeling from New England Biolabs Cancer Research from Sinai Health System...
  31. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit in the extended...
  32. The Pleiades Promoter Project

    Type
    Collection
    ...Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra, V., Docking, T. R., Dreolini, L....
  33. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...recombinant monoclonal antibodies or R-mAbs have been engineered such that they are a distinct IgG subclass from...expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens in a new...
  34. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...2014. PubMed PMID: 24151867 Mikkelson, et al. Metab Eng. 2012. PubMed PMID: 2232647   SapTrap, a toolkit...genome engineering across multiple species Recent discoveries in the fields of genome engineering and synthetic...pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3...fold a protein because of its length. Mark Howarth’s lab recently engineered a new peptide/protein pair,...as not only a protein engineering tool, but also as a way to characterize/engineer promoters, enhancers... developed for the study of enhancer function, strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...
  35. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...ways of delivering healthcare. Clients typically engage consultants as short-term experts to work on “cases...Cases present specific operational or commercial challenges or questions that clients face imminently. Consulting...on analyzing the situation that presented the challenge at hand and help resolve it through well-informed...specialize in business strategy tend to be mostly engaged by pharma and biotech clients. In this realm, there... there are a few different kinds of challenges that consultants get called upon to tackle, depending on...to collaborate whenever it faces an important challenge. The types of projects mentioned above are just...rewarding parts of the job is being confronted with challenges that are crucial for the success of a drug’s ...
  36. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...accomplish is to make the engineering of biology much easier. So you could imagine engineering the entirety of ...dig into its engineering qualities, what these parts allow is for someone to forward engineer a system of...Drew Endy's lab at Stanford develops engineered DNA systems capable of storing data and computing inside...an organization working to advance biological engineering openly so as to benefit all people and the planet... to not assert property rights for particular engineered genetic sequences; but it’s only the promises... what we think of as an operating system for engineering an entire cell. Typically when people use plasmids...of a cell. That's a big long-term technical challenge. We have a strategy that organizes how we start the...
  37. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...in more genome engineering technologies? Browse others on Addgene's Genome Engineering Guide. Fire ...gene targeting Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that can be used to...biochemical characterization, has enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence...transcriptomes, as well as further optimization through engineering, Ed Boyden’s lab has identified a variety of ...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate...field of optogenetics. Using a rational protein engineering approach, they designed Opto-RTKs, which activate... has now made improvements to this system by engineering cells that lack release factor one (RF-1; Bacterial...
  38. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...located in a range of wavelengths of about 380–700 nm. Photons of lower wavelengths are higher in energy...while photons of higher wavelengths are lower in energy. As such, wavelengths below 380 nm lie within ...can do so at a range of wavelengths, rather than at just one defined wavelength. Likewise, the emitted ...within the UV spectrum, while wavelengths over 700 nm make up the infrared (IR) spectrum. When photons are ...energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher than those of absorbed...emitted photons also appear within a range of wavelengths. For that reason, we speak of excitation and emission...APC-Cy7. Conjugating Cy7 to APC results in a higher-wavelength emission spectrum compared to APC. This happens...
Showing: 101 - 150 of 808 results