...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted... This system uses a 10-day protocol, generates “clean” homozygous mutants with no co-integrated markers...

...cells with single vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed (Link opens...Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala. Nat Neurosci. 20(12):...

...e.g. converting a DNA binding protein to a DNA cleaving enzyme) or to regulate the activity of a protein... by tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair ...

...AGCACTGTTGCCGGCCGAGG 79006 cut S. pyogenes 26944679 Shaw Please refer to the primary article to confirm details...add validated gRNA sequences to our datatable, please download the following spreadsheet, fill out as...

...plasmids you would like to see added to this list, please email [email protected] . Synthetic Biology Plasmids...

...Addgene as well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody...

...Addgene as well as by our trusted partner labs. To learn more about antibodies and their use, visit our Antibody...

...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...

...create fusion proteins with your gene of interest. Please visit Michael Davidson's Molecular Expressions ...

...sharing our protocols and quality control methods. Learn More Guide New to using antibodies? In our Antibody...

...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...

... at specific locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...protein-protein interactions in vitro and in vivo . Learn more about in vivo imaging tools in this blog post...

...scFvs, DARPins, Fabs, and secondary antibodies. To learn more about antibodies and their use, visit our Antibody...

...grouped together as part of a pathway or ORF. MAPK Nuclear Receptors Find a Pathway or ORF Kit Disease Research...

... unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically along the...

...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...

...targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute Partnership ...

...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy chain...promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...Addgene as well as by our trusted partner labs. Learn More scFv Plasmid Collection The following plasmids...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles...

...translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat ...

...Check out our Viral Vector Packaging Service and learn about our options for hundreds of in-stock preps...

...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...