We narrowed to 1,015 results for: LEA

...catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand...

...sharing our protocols and quality control methods. Learn More Guide New to using antibodies? In our Antibody...

...tables below for CRISPR interference plasmids. To learn more about CRISPR interference and other CRISPR ...

...as well as academic and nonprofit labs. Want to learn more? Read our blog post about this collection ,...

...for CRISPR plasmids designed for RNA editing. To learn more about RNA editing and other CRISPR topics, ...

...tables below for CRISPR activation plasmids. To learn more about CRISPR activation and other CRISPR topics...

...plasmids designed for epigenetic modification. To learn more about epigenetic modification with CRISPR and...

...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...

...designed for the purification of genomic loci. To learn more about purification using CRISPR and other CRISPR...

...designed for the visualization of genomic loci. To learn more about visualization using CRISPR and other ...

...selective imaging of tissues in living animals. Learn more about in vivo imaging tools in this blog post...

...scFvs, DARPins, Fabs, and secondary antibodies. To learn more about antibodies and their use, visit our Antibody...

...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...

...and how to use these tools in your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids...

...targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute Partnership ...

...tables below for CRISPR base editing plasmids. To learn more about base editing and other CRISPR topics,...

...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

...promoter for heavy chain expression, and an ER signal/leader sequence (L) for translocation of the heavy chain...promoter for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...Addgene as well as by our trusted partner labs. Learn More scFv Plasmid Collection The following plasmids...

...carefully optimized for both safety and efficiency. Learn more about the different types of viral vectors ...

...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...

...translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic I. Nat ...

...assembly of up to three transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway...

...large reduction in tumor suppression activity, leading to the development of tumors in early adulthood...

...synthesis via partial UAG codon reassignment and release factor 1 deletion . FEBS Letters , 586 (20), 3716...

...://doi.org/10.1089/hum.2019.277 Lavin TK, Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success...BioRender.com   Causes of recombination and leaky expression While leaky expression and spontaneous recombination...will have some level of recombination that can lead to leaky expression in cells that do not express Cre ...scrambled cre-recombinase sites lead to greater frequency of reversion and leaky expression. On the right non-homologous...recombination between these sites may be the cause of leaky expression. To test their hypothesis, they randomly...recombination. This indicates that the amount of leaky expression can largely be attributed to the level... non-homologous scrambled recombination sights lead to reduced reversion and less off-target expression...

...RuvC-like endonuclease domain, but they lack Cas9’s second HNH endonuclease domain. Cpf1 cleaves DNA in ...orthologs that display cleavage activity in mammalian cells. Like Cas9 nucleases, Cpf1 family members contain...based system, require a single-component nuclease to mediate cleavage rather than the multi-subunit complex...contains a RuvC-like endonuclease domain, but it lacks Cas9’s other HNH endonuclease domain, indicating ...functional CRISPR nuclease that could be adapted for genome editing. The use of a different nuclease could potentially...that display robust cleavage activity similar to that of Cas9. These two nucleases, AsCpf1 and LbCpf1 ...proximity to the cleavage site, preventing future edits. In contrast, since Cpf1 cleaves relatively far ...

...Cas13a. The origins of Cas13a: An RNA cleaving CRISPR nuclease Cas13a was originally identified in 2015... crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now...domains to cleave target DNA. The HEPN domains within Cas13a are essential for RNA cleavage, consistent...bind and cleave RNA non-specifically. The ability of dCas13a to bind target RNA without cleaving the molecule...between Cas13a and Cas9 is that Cas13a binds and cleaves RNA rather than DNA substrates. In terms of structure... residues in the Cas13a molecule results in a “nuclease dead” Cas13a (dCas13a), that is capable of binding...binding target RNA but lacks the ability to cleave the RNA target.  Table 1: Comparison on Common CRISPR...

...gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently...fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence and this approach... This activation results in a trans cleavage reaction that cleaves a nearby RNA sequence that contains...activated and performs non-specific collateral cleavage. This cleavage is coupled to fluorescence visualization...fluorescently labelled ssDNA substrates. Upon cleavage, the fluorescence is no longer quenched and can be ... a PAM (Protospacer Adjacent Motif) for target cleavage. This test can be performed in two steps for challenging...ssDNA-FQ reporters that exhibit fluorescence upon cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA...

...) to cleave ssRNA or mRNA. It also exhibits a “collateral effect” of non-specific ssRNA cleavage. How ...ssRNA cleavage byproducts. Thus, binding of Cas13 to the programmed region of interest would lead to both... the Addgene Blog Learn about using toehold switches to detect Zika virus   Learn more about targeting...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen...Both SHERLOCK and DETECTR harness the promiscuous cleavage and degradation of neighboring ssRNA and ssDNA...ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable signal from this...to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules. In SHERLOCK a quenched...

...for Cas9 nuclease function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they...the two nuclease domains, Cas9 ensures both are positioned correctly before HNH or RuvC can cleave DNA, ...? It’s not actually clear! In vitro, Cas9 holds tightly to DNA even after cleaving it, so many researchers...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature Biotechnology...   Cas9 has two major lobes, called NUC (for nuclease) and REC (for recognition). The NUC lobe contains... and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal ... and helps recognize the guide RNA. Unlike the cleavage domains, the recognition domains—including the...