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We narrowed to 235 results for: crispr grnas

Showing: 121 - 160 of 235 results

  1. CRISPR Plasmids - C. elegans

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR C. elegans CRISPR Plasmids: C. ...following CRISPR plasmids have been designed for use in C. elegans. Cut Fully functional CRISPR/Cas enzymes...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors... When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same...

  2. CRISPR Plasmids - Drosophila

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors... When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in the same...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

  3. CRISPR Plasmids - Bacteria

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes... Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...

  4. CRISPR Plasmids for Genomic Visualization

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Visualize CRISPR Plasmids:...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...

  5. CRISPR Antimicrobials

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    Blog Post
    ...Plasmids for Your Research Read Our CRISPR Guide Pages Find CRISPR gRNA Design Tools .... Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over traditional...plasmid-based, CRISPR systems (Find the Citorik Plasmids here). In both cases, they used 1-2 CRISPR crRNAs targeting...strategies for bacterial CRISPR targeting. Phage-derived vectors carry CRISPR RNAs (crRNAs) targeting ... Blog CRISPR Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing...Using CRISPR to Edit Disease out of the Genome  Additional Resources on Addgene.org Find CRISPR Plasmids...’ll have less potential to become resistant to? CRISPR may provide a method for doing just that. While...

  6. CRISPR Plasmids - Plants

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes...Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...

  7. CRISPR Plasmids - Purify Genomic Loci

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Purify CRISPR: Purify Genomic ...genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification...region of interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  8. CRISPR Plasmids - Tagging

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...

  9. CRISPR Plasmids - Parasites

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Toxoplasma CRISPR Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library... CRISPR Parasites CRISPR Plasmids...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and...

  10. CRISPR Plasmids - Repress Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Interfere CRISPR Plasmids: Interfere...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer...

  11. CRISPR Guide

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    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...basic CRISPR mechanism Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...

  12. CRISPR Plasmids - Activate Gene Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR... CRISPR Activate...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter...

  13. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR CASTs CRISPR Plasmids...Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick ...plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large...BioRender.com. Browse, sort, or search the tables below for CRISPR Transposase plasmids. Plasmids are available for...

  14. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Nick CRISPR Plasmids: Nick...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...Nick Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  15. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...

  16. CRISPR Plasmids and Resources

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    ...Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and the basics...planning your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've... for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more. CRISPR History...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on Addgene's... are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries for knockout...Visualize CRISPR fluorescence methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...

  17. CRISPR Plasmids - dCas9-FokI

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  18. CRISPR Plasmids - Epigenetics

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and... dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your...and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  19. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR Cut CRISPR Plasmids: Cut Browse CRISPR...CRISPR plasmids that create double strand breaks in DNA. CRISPR... Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  20. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    ...Microbial CRISPR diversity is impressive, and researchers are just beginning to tap the wealth of CRISPR possibilities...Cas13d CRISPR array processing is robust, with CasRx and either an unprocessed or processed gRNA array ...RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History  ...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes ...general bioinformatic screens that looked for a CRISPR repeat array near a putative effector nuclease....these papers can independently process their own CRISPR arrays into guide RNAs. crRNA cleavage is retained... WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity ...

  21. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ...comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s...it won’t tell you whether the vector contains the gRNA insert or not. A blue-white screen, however, can...and distinguish between plasmids that contain the gRNA (white) from those that do not (blue). Let’s take...

  22. CRISPR Plasmids - Empty gRNA Vectors

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...genome. gRNA Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector...Empty gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas ...

  23. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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    ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type...needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also...variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities Dimeric CRISPR RNA-guided FokI Nucleases... Nucleases (RFNs) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression ...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids Genome...

  24. Plasmids 101: Screens vs. Selections

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    ... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ... With the advent of CRISPR, genome-wide knockout screens are possible with gRNA libraries targeting all...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest... be lethal to cells and thus not assayable with CRISPR. Another method, popularized in yeast, are temperature...

  25. CRISPR Plasmids - RNA Editing

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila... CRISPR RNA Editing CRISPR Plasmids...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity...

  26. CRISPR Pooled gRNA Libraries

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664...ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries ...plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target...experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic...

  27. CRISPR Plasmids - RNA Targeting

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In ... of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity... . Browse, sort, or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available ...

  28. CRISPR Plasmids - Base Edit

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR plasmids for precisely editing DNA bases without inducing a DNA break. CRISPR... CRISPR Base Edit CRISPR...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which Cas9 is fused ...

  29. CRISPR Plasmids - Prime Edit

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Browse CRISPR Plasmids By Function ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that... . Browse, sort, or search the tables below for CRISPR prime editing plasmids. Plasmids are available ...

  30. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ... Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout...Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently...lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral... in experiments other than just those involving CRISPR and so we also have lentivirus for delivering control...

  31. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment Suggest A Plasmid You'd Like... Type Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “... expression, plant expression, mouse targeting, CRISPR, TALEN, synthetic biology, affinity reagent/antibody...

  32. CRISPR 101: Cytosine Transversion Editors

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    ... which. Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a...tip! In CRISPR, the non-target strand is the DNA strand that does not base pair with the gRNA. Base editors...blog CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: ...Editing CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids...editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...base editing tools First, let’s briefly review how CRISPR-derived base editing works in general. Base editors...

  33. Hot Plasmids and Viral Preps - March 2021

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    ...very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex...       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic...Saptrap cloning systems to generate AID* tagged CRISPR knock-in repair constructs. These repair constructs...tagged AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of ...Cas9 cleavage can induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine...

  34. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post
    ...Most popular methods for CRISPR activation CRISPR activation uses dCas9, a CRISPR protein variant lacking... blog Browse our many CRISPR blog posts Get the basics of CRISPR in the CRISPR 101 blog series Resources...making it a common choice for CRISPR activation. Newer methods for CRISPR activation While SAM, SunTag,...compared to other CRISPR activators, but their initial results show promise for CRISPR-based gene activation...Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...Prior to the discovery of CRISPR/Cas systems, gene activation across multiple loci was an arduous process...activation seem next to impossible. The development of CRISPR/Cas systems, however, greatly improved the simplicity...

  35. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...expanding CRISPR beyond spCas9 Cas proteins More resources on the Addgene Website CRISPR Plasmids ...engineering workhorse You rarely hear the word ‘CRISPR’ without shortly thereafter hearing ‘Cas9’. The...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...targeting. RNA targeting Cas enzymes Move over Cas9, CRISPR isn’t just for DNA anymore! Cas enzymes have been...Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019). ...is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016...

  36. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...yeast CRISPRa plasmids here Reduce the scale of your CRISPR screens by using this lentiviral CRISPR human...for use in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items ...Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox with their...sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...chemically inducible CRISPRa system in yeast is mediated by recruitment of MS2-functionalized gRNAs.  Find the...their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of huge phages. ...

  37. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely...2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method to tag endogenous proteins in...and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein...call the LADL Anchor. Using specifically designed gRNAs, they can then recruit these LADL Anchors to genomic...

  38. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...up-to-date information. Check our CRISPR guide and blog to learn more about CRISPR and the most recent developments...prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd generation...original lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents...by delivering shRNA or miRNA. The ever expanding CRISPR field makes wide use of retroviral delivery systems...systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening of ...expression, gene knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression...Vector provided by the Qi lab. Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral...

  39. CRISPR History and Development for Genome Engineering

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    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no...information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced...prokaryotes used CRISPR as part of an adaptive immune system - utilizing various CRISPR-associated (Cas...

  40. The AAVantages of AAV in CRISPR Screens

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    ...Limitation for CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using...development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily customizable...resources at addgene.org CRISPR pooled libraries Guide to pooled libraries CRISPR guide AAV guide ... interference, activation, and knockout. Most CRISPR screens rely on lentiviral systems, a fact supported...supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large packaging... AAVantageous!       In vivo, in vitro, ex vivo CRISPR screens can be carried out using a few different... process of these screens is the same: Deliver CRISPR library to cells of interest. Select for cells ...
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