We narrowed to 355 results for: Cre expression vector

...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...AAV vectors offer some advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...

...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...

...Edit Collection The CRISPR Guide Empty gRNA Expression Vectors... we performed high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled ...cell contexts. Figure 1: High-throughput screening of pegRNA efficiency and building prediction tools...sequence-based prediction models from self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights...your target. Figure 3: High-throughput screening of the effect of chromatin on prime editing efficiency...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor... none Cre dependent 1 Kaspar Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent...none Cre dependent 1 GENIE Project 234442 pAAV-syn-flex-iGluSnFR4f-NGR-WPRE Syn iGluSnFr4f none Cre dependent... none Cre dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent...pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a GCaMP6m none Cre, Flp and VCre dependent 8 Karl Deisseroth Calcium Sensor...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under...

...along with Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal...on the Addgene Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

...cell type-specific expression. The Allen Institute for Brain Sciences has screened thousands of enhancers...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...

...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help...iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and...

...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently integrated...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors: Marjorie Buss and Mengtong "Tom" DuanVisualizing...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral Service...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti...

...Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma and... E. coli expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier...- Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc - Lindquist...Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources : Find...plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied as one item. ...Santalucia Labs pCri System : Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth...constructs - Lahaye Lab Vaccinia virus ORF library : Expression library that contains each of the predicted open...

...Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...research, visualization, protein expression, synthetic biology, and viral vector plasmids. Plasmid...to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant phosphoproteins...Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank for feeding...continues to be challenging. Organism specific viral vector systems would also be beneficial for many systems... progress on the NSF “G to P grand challenge.” Creating a productive, new scientific community is no small...in particular, that they all share the plasmids created under this programs via Addgene. New organisms ... organisms) -  perhaps you’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project...crisis. I like how they are using protocols.io to create a protocol community! Asclepias syriaca and Asclepias...used to study aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black footed slipper...

...with natural chain termination at stop codons, expression levels of these tagged proteins are often very...could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA ...intact cells. The Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence...

... Bac-to-Bac Expression System, AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with...cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine...SGC Toronto SGC Oxford SGC Stockholm Bacterial Expression Plasmids (empty backbones/controls) ID Plasmid...Thrombin cleavage, Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR ...hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: www.thesgc.org...

... whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A...better detect dim structures or low levels of expression with the same imaging workflows you already use...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility...no wonder that self-labeling protein tags are increasingly popular. Embrace the flexibility and switch ...

... 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX...pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B...101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag ...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...Synthases (AcpS and Sfp, respectively) may be expressed and purified as described previously in Stachelhaus...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies...

...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for detecting ... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful for both intracellular...want minimal disruption of endogenous protein expression and interactions, such as exploring viral biology...HaloTag technology is optimized for various protein expression systems including E. coli , mammalian cells, ...interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two proteins interact,...

...cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide is typically...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...

...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...Return to top Additional Resources Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew...and codon-optimized the Open Enzymes parts for expression in Escherichia coli . Researchers can use the...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

...Transl Med. 2013) Express human DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with...Science Collection . Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. Study...Flag tag. (Bennett et al. Heliyon. 2020) Use VAP expression plasmids to study ER contact with other organelles...Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer...Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors...

... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...another round. Figure 1: Overview of prime editing. Created with BioRender.com. The original prime editing ...

...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

...MK, Elfinger M, Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...generated by SnapGene software.  Thanks to increasing affordability and accessibility, more researchers...by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies to isolate the monomer Sometimes... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...DNA-forming sequences and WRN deficiency independently increase the frequency of base substitution in human cells... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Additional... Additional Resources Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...

...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit ... our website. Placing an Order: After you have created an account, you can place orders through our website...

...for many molecular biology protocols, including vector-insert joining for recombinant plasmid construction..."Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog...reliably detect single-base differences. The original creators of this technique visualized the ligation product...quencher pairs or qPCR-based methods to greatly increase the sensitivity of detection (14-20). LDR has ... 27). This methodology has been used to rapidly screen buffer conditions for Taq DNA Ligase and their ...electrophoresis (CE) (see below). This method allowed screening of all possible base combinations (Watson-Crick...of a 96-well plate, allowing 6 conditions to be screened per plate. The results indicated that the optimal...

...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms.... PMID: 36893201 (Link opens in a new window) Increasing access for biochemistry research in undergraduate...

...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria...CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila. Mammalian...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... gRNAs will be expressed in the same cell. This is called multiplexing , and increases the likelihood ...lentivirus or use a Cas9-expressing stable cell line. After applying your screening conditions to your transduced...also been used for genome-wide screens to activate or repress gene expression in mice and human cells. The...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...

.... PubMed Central PMCID: PMC4523203. Many empty vectors containing these fluorescent proteins are available...available on our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids...biosensors too, as another laboratory may have already created the sensor that you need. FRET biosensors designed... endogenous state that you want to measure (overexpressing a gene or protein after transfection). Future...multiple FRET biosensors simultaneously would further increase the amount of correlated information available...

... by expressing and purifying the phosphosite library as GST-fusion peptides (“Mode #1” expression). The...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...characteristics, and an overall improved ease of use when expressing recombinant phosphoproteins. These attributes... origins. Therefore, when choosing plasmids to express the phosphoprotein of interest, a medium copy RSF1030...2023) and find general tips on phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli...phosphorylation-dependent protein-peptide interactions (“Mode #2” expression, Barber et al., 2018). The phosphosite library...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...

...3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 0.51 530... 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...

...protein translation machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS enzyme...make sure that these conditions are true. Always express first with a control reporter gene: GFP for E. ...mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI Return to top Bacterial Strains Browse ...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...197656 BL21(DE3) ΔserC serC gene knocked out; for expressing proteins containing site-specific non-hydrolyzable...

... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute carrier...

...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...-generation rAdV vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation...third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with BioRender.com. Third-generation...Third-generation rAdV vectors and helper virus. Created with BioRender.com. Adenoviral Vector Production Cloning...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

...Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for...assembly of expression vectors for multi-subunit protein complexes in various hosts. Expression cassettes...Toolkit Mammalian Expression Yizhi Cai 95 plasmids to generate mammalian expression vectors for transient ...Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 plasmids...protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression Brigitte...

...Viral Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV vector production. Created with BioRender.com...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...

...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...AAV vectors offer some advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. Empty Backbones , ... protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian, yeast...inserted gene. An expression vector can also include an enhancer sequence which increases the amount of protein.... In expression plasmids, the MCS is often located downstream from a promoter to drive expression of the...control the level of insert expression, as a strong promoter directs high expression, whereas weaker promoters..., Cloning Kits Expression Plasmids Used for gene expression, usually for the purposes of gene study. Expression... Expression vectors must contain a promoter sequence, a transcription terminator sequence, and the inserted...

...Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...Overview of lentiviral vector production. Created with BioRender.com. Viral Vector Integration Genome-wide...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...

...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...fiber. Using viral vectors for opsin delivery results in more localized expression of the opsin. Depending...specific recombinase technology like Cre-lox, which allows you to express an opsin ONLY in a genetically defined...site of the viral vector, rather than in all cells. For example, using a viral vector with a floxed opsin...opsin in a VGAT-cre animal will result in expression of the opsin only in inhibitory neurons near the injection...procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of...as those described below, can be targeted and expressed in specific subsets of neurons, allowing precise...

...Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols... Gamma-retroviral vector packaging methods. Created with BioRender.com. Viral Vector Integration Much ...Gamma-Retroviral Vectors Vector Production Integration Pseudotyping Common Uses Safety Resources Plasmid ...long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...

...chemogenetic receptors. FLEx vectors block expression in the absence of Cre and are especially useful in AAV... Dlx Interneurons EF1a, CAG General expression Targeted Expression Depending on the experiment, an AAV-encoded...which they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic.... The opsin may excite or inhibit the neurons expressing the LMO, depending on its biophysical properties...more than one domain, both of which need to be expressed. However, neuronal control through PSAMs and LMOs...) is a widely-used tool for achieving in vivo expression of chemogenetic receptors. Check out Addgene'...