...Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. ...systems. Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. Nucleic Acids Res. 2013 Mar 4. PubMed...

...multiple subunits or isoforms, individual links to each gene page are provided below. Color is used for ...multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1...

...is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis from the T7 promoter...Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used to create...

... of this page. For more in depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids... Capsids . To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and...

...elegans , mouse, human, rat, yeast, frog, Brachypodium distachyon, Oryza sativa, Oryzias latipes CasFinder...

...translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery are ...

...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

... GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...31255 pGEX-4T2 Parkin RBR PRKN GST tac Alzheimer's Rachel Klevit 31606 pHA-deltaN335-Kin1A KIF5A HA CMV ALS...Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448 pEGFPC1-hVAP-B VAPB GFP, HA CMV ALS...pHH0103_PFN1_Profilin PFN1 GST tac ALS Sachdev Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His...

...their career choices.” She points out that a PhD teaches analytical and critical thinking skills that are...on to work in patent law, business development, teaching, etc. Church also encourages his mentees to follow...passion True success evolves from heart and hustle. Each PI emphasizes the importance of passion, hard work...

...experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA...

...experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA...

...experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA...

...Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each encoding a single TALE repeat domain and flanked...

... by cloning a pair of annealed oligonucleotides (each 26 nts in length) into BsaI-digested pDR274 vector...

...teams of 4 people face each other on a single chosen clinical disease case. Then, each team received a whole...planarian tools for teaching developmental biology Learn how BioBuilder can help teach students about Synthetic...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation...knowledge to solve a clinical disease case. As a teacher, you explain the game rules and then you let your...Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected card... 10 minutes to expose their strategy, justifying each single element used to build the construct and explain...practice, so it is based on a win-win strategy. After each team presented, all students had to pick the best...

... by cloning a pair of annealed oligonucleotides (each 26 nts in length) into BsmBI-digested MLM3636 vector...

...stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer...

...the delivery of the synthetic guide RNA (sgRNA). Each of the Cas9 vectors contains the Cas9 coding sequence...

...-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and ...

... transparency and accessibility in research. For each antibody, we provide important details including...