...standard polymerase protocol), causing the enzyme to perform exonuclease activity until the first "G" in the...sterile dH20 to 40 μl Step 4: Amplify Insert by PCR Perform PCR amplification of your insert following the ...your PCR product before proceeding, as they will interfere with the exonuclease activity of T4 Pol in the...

...phone-sized, foldable microscopes that aren't very powerful (but are cheap and accessible) to massive transmission... are multiple objective lenses. Each objective performs essentially the same task but has different magnifying...scratches, and dust can easily impair microscope performance. Be sure to clean up your work area and microscope...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

... of a stable cell culture after transduction. Performing antibiotic selection on transduced cells enables...5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells...untransduced well (0 µL lentivirus, above) are dying. Perform regular fluid changes the monitor the growth of...

...follow the kit's instructions. If you want to perform plasmid purification without using a kit, you can...ribonuclease that digests single-stranded RNA. (Optional) Perform phenol-chloroform extraction - see protocol below... DNA purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant...

...Watch the protocol video below to learn how to perform Polymerase Chain Reaction (PCR). Basic PCR Program...pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: Continued...reagent by the number of individual PCRs you wish to perform and add 10% extra to account for pipetting error...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...programmable endonucleases that can be used to perform targeted genome editing. RGNs consist of two components...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...fusion protein. In Vivo Imaging Image with these powerful tools to study individual plasmids or protein-...

...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from from the initial stable...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...

...localization Optogenetics Background In vivo imaging is a powerful tool used to study individual plasmids or protein-protein...