...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase...recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

... nLightR. A, Structural models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita...indicators: nLightG and nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR...al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...norepinephrine. nLight is 28-fold more selective than GRABNE, with both nLightG and nLightR showing significantly...experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly expand the toolbox... both nLightG and nLightR, and may soon have a red tool which matches the sensitivity of nLightG. Many...images of HEK293T cells and neurons expressing nLightG or nLightR before/after application of NE (10 μM) and...

... clones for loss of function. This is due to the high frequency of indels formed on non-deleted alleles...cells in order to enrich for cells that received high levels of the CRISPR/Cas9 constructs. Plate sorted...the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nature Protocols...

...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

...Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids....

...combination allows IPI to generate antibodies to highly conserved proteins and other difficult-to-target...part of that mission, IPI is providing access to high-quality, well-characterized antibodies in an open...

...of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster...nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster...

... the results, techniques and reagents with the highest-possible quality of research output, RESOLUTE expects...production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated...

...a variety of popular plasmids and backbones with high cloning potential. By making these plasmids available...spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations. Can I directly transfect...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...Promoter 107787 AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Light-Inducible Recombinases ID Name Promoter Fluorophore...

...Request Viral Service: AAV Made on Request Due to high demand for viral items, Addgene's AAV Made on Request... Price $3030 USD plus shipping Turnaround Due to high demand for viral items, Addgene's AAV Made on Request...

... biological materials and intellectual property rights, and provides liability protection for material...qualified for-profit entities. Addgene reserves the right to determine if an entity is qualified to receive...

... plasmid with or without expression of Cas9. Highlighted below are some multiplex gRNA vectors from our...Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and...

...pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling Browse...Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and...

...their large packaging capacity (>8 kb), high titers, and high levels of transgene expression. Additionally...

...γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection results...pRXTN CMV/MSV pRXTN is a modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 ...

...Method Full Target Site (5' to 3') Left half-site Right half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg...engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol...

... 87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP...

...consider when planning experiments). What resources might be useful to help improve this technology or its...are some current obstacles that researchers face right now (e.g., not enough protocols, cost, not enough...