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Showing: 41 - 60 of 666 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches...MF, Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition...26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that inactivate ...132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-...Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015. Engineered...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...as early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where...where symptoms appear during or after a person's mid-sixties. The early-onset form accounts for less than...created with support from the BRAIN Initiative. Jackson Laboratory collection of neurological mouse models...Foundation is dedicated to finding a cure for Parkinson's disease through an aggressively funded research... of improved therapies for those living with Parkinson's today. The Michael J. Fox Foundation has made...

  3. Lentivirus ddPCR Titration

    Type
    Protocol
    ... two copies of RPP30 in diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...

  4. Brain Initiative Collection

    Type
    Collection
    ...105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the ...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP ...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics...Chemogenetics expression plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics...Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A....

  5. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... and Cap7 Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 Wilson 112865 pAAV2...not known to cause disease in humans. For these reasons, AAVs are generally contained at lower biosafety...packaging, expressing adenovirus E4, E2A and VA Wilson RepCap Plasmids for Serotypes Available as a Viral...AAV packaging plasmid, expressing Rep2 and Cap1 Wilson 104963 pAAV2/2 AAV2 AAV packaging plasmid, expressing...AAV packaging plasmid, expressing Rep2 and Cap9 Wilson 112866 pAAV2/rh10 AAVrh10 AAV packaging plasmid...plasmid, expressing Rep2, and rh10 capsid Wilson 81070 rAAV2-retro helper AAV retrograde AAV packaging plasmid...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664, 177670 Inhibition Human Adamson 3rd ∼3 366... 131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 ...Tan 2nd 4 on average 94,000 Bradley Human CRISPR Poison Exon Knockout Library 138084 Knockout Human Bradley...

  7. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...1998 References George N, Pick H, Vogel H, Johnsson N, Johnsson K. 2004. Specific labeling of cell surface...9712910 Vivero-Pol L, George N, Krumm H, Johnsson K, Johnsson N. 2005. Multicolor imaging of cell surface... England Biolabs website. For a comprehensive comparison to GFP, please refer to this table. Technology...

  8. Luciferase Plasmids

    Type
    Collection
    ...Luciferase complementation in plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular...co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267 RedLuc/Gaussia Creation of C-...Gaussia luciferase for ratiometric imaging David Nelson Return to Top Expression Constructs Luciferase ... expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia... CMV AAV expression of firefly luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression... expression of firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian expression...

  9. Antibody Guide

    Type
    Collection
    ...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

  10. Genetic Code Expansion

    Type
    Collection
    ...Bacterial Jason W Chin 174515 pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA) PylRS M. mazei Bacterial TGA Jason W Chin ... AfTyrRS CbzK and p-I-Phe Bacterial TCG and TAG Jason W Chin 105829 pIRE4-Azi Azi-tRNA synthetase (EAziRS...coumarin-yl) ethylglycine Bacterial TAG Kenneth Johnson 82417 pUltra-sY sY-specific aaRS M. jannaschii ..., TCA, or TAG codons in all open reading frames Jason W Chin 174514 Syn61Δ3(ev5) No TCG, TCA, or TAG codons... codons. Deletion of serT, serU, and prfA genes Jason W Chin 189857 Syn61Δ3(ev5) ΔrecA (ev1) No TCG, TCA...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Structure Plasmids mCrimson3 588 615 - Monomer mCrimson3-N1 - Mammalian Expression mCrimson3-C1 - Mammalian...FRET Biosensors Subcellular localization Michael Davidson Collection Blog: Which FP Should I Use? Blog: ...Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal... GFP - C-terminal GFP for bacterial expression Davidson Lab Plasmids - Includes many fluorescent proteins...

  12. Rett Syndrome

    Type
    Collection
    ...Link opens in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for ...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link...window) PMID: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts...patient-derived iPSCs (Link opens in a new window) Jackson Labs - mouse line developer and repository, and...Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...patient-specific or disease-specific cells. For this reason, iPSCs have become an excellent unlimited stem ...Cells. Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression...piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system; expression of mouse Oct4... pii: cells9112451. doi: 10.3390/cells9112451. Ottosson Stem Cell Research Plasmids This table contains...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...permuted fluorescent protein (cpFP) or Förster resonance energy transfer (FRET) efficiency of a pair of...locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and small molecule... levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer sensors for quantitative monitoring... Science. 2018 Sep 14;361(6407):1122-1126. Kai Johnsson NADPH/NADP+ or NAD+ Snifit FRET-based sensors ...dinucleotides. Elife. 2018 May 29;7. pii: 32638. Kai Johnsson Phosphate Scaffold for inorganic phosphate biosensor...Am Chem Soc. 2012 Nov 21;134(46):19026-34. Kai Johnsson Glutamate Third generation glutamate sensors (...construct custom biosensors. The ScEnSor Kit from the Olsson laboratory includes eight fluorescent reporters...

  15. Caltech Systemic Capsids

    Type
    Collection
    ... CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry...Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 140135 pAAV-EF1a-...

  16. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve ...(6). PMID:31636080 Wick ZC, Tetzlaff MR, Krook-Magnuson E. 2019. A novel population of long-range inhibitory...Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan C,...Hyun M, Umadevi Venkataraju K, Straub C, Wang W, Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal...

  17. Retrograde AAV viral preps

    Type
    Collection
    ...expression Recombinases Wilson 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases Wilson 55634 pAAV-EF1a-mCherry-IRES-Flpo...105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson Recombinases 24593 AAV-pgk-Cre PGK Cre expression...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn EGFP-tagged Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...

  18. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...relevant to Parkinson's disease created by the Michael J. Fox Foundation... Plasmid Collections Parkinson's Disease...along with individual laboratories to assemble a Parkinson's disease plasmid resource for the scientific research...

  19. Depositor Collections

    Type
    Collection
    ...Collection Visualization Michael Davidson Fluorescent Protein Collection - Davidson Allen Institute for Cell ...mini-promoters to drive selective expression in the brain - Simpson Reagents for improved expression of recombinant...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...like transcription. Michael Davidson FP Collection Browse the Michael Davidson comprehensive collection ...UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra Database hosted at the University...
Showing: 41 - 60 of 666 results