...staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...vector preparation. In some cases, in vivo testing of these viral preparations was performed by the Karpova...

...secreted version of the protein is available – these attributes have made it popular with scientists since its...luciferase reporter vector via Gateway cloning to test for the presence of transcriptional regulatory region...

...DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about temperature? Find... Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions, but what about...

...Reddy TE, Gersbach CA (2015) Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes... M, Safi A, Song L, Hilton IB, Crawford GE, Reddy TE, Gersbach CA (2017) CRISPR–Cas9 epigenome editing...across the genome) has been implicated in disease states, and targeting the epigenome may make certain processes...plasmid form or as ready-to-use lentivirus. Tet1 initiates cytosine demethylation of DNA. However, several...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers. Although the lab used this... a transcriptional activator like VP64 or VPR activates transcription, whereas dCas9-KRAB fusions repress...

...Thakore PI, Perez-Pinera P, Brown MT, Majoros WH, Reddy TE, Gersbach CA. Correction of dystrophin expression...Ousterout DG, Kabadi AM, Thakore PI, Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome ...grip strength, specific force, and other muscle tests, muscle function is clearly improved, although not...at the ten highest-ranking predicted off-target sites. Each paper characterizes additional, unique facets...

... represent the official views of the National Institutes of Health....

...designed for efficient nuclear localization in eukaryotes....

...Nuclease ( RGN ) technology to modify three different sites in the EGFP reporter gene (Fu et al., Nat Biotechnol...

...and terminators repressors and activators logic gates Networks and Gene Regulation Plasmids Search the...

...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

...blocking ligand binding and receptor function Figure courtesy of IPI Available Antibodies ID Recombinant Antibody...

...Nuclease ( RGN ) technology to modify nine different sites in various endogenous zebrafish genes (Hwang & Fu...

...single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These 28 plasmids...

...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

...targeted to a sequence of interest. Potential target sites can be identified using the publicly available, ...

...in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease domains...

...to enable identification of potential RGN target sites and to facilitate design of short oligonucleotides...

...lab can be used both to identify potential target sites for RFNs and also provides the sequences of oligonucleotides...

...for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential for regulating...