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We narrowed to 35 results for: human dat

Showing: 1 - 35 of 35 results

  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...N222/6R] Clcn4 Human Mouse IgG2a 114475 Anti-PARIS/ZNF746 [N196/16R] PARIS/ZNF746 Human Mouse IgG2a 114476...Mucolipin-3 Human Mouse IgG2a 114478 Anti-GABA(A)R, Epsilon [N413/67R] GABA(A)R, Epsilon Human Mouse IgG2a...Anti-Stonin-2 [N346/9R] Stonin-2 Human Mouse IgG2a 114485 Anti-NSD3 [N348/82R] NSD3 Human Mouse IgG2a 114486 Anti-Flrt3...Menke's disease protein) Human Mouse IgG2a 114503 Anti-LRRK1 [N341/37R] LRRK1 Human Mouse IgG2a 114504 Anti-TrpC4...Anti-Malin [N85/18.1R] Malin Human Mouse IgG2a 114514 Anti-DNMT3L [N117/9.5R] DNMT3L Human Mouse IgG2a 114515 Anti-GABA...N147/6.1R] Pan-QKI Human Mouse IgG2a 114525 Anti-FGF13/FHF2 [N91/27.1R] FGF13/FHF2 Human Mouse IgG2a 114527...1.1R] Neurexin-1-Beta Human Mouse IgG2a 114533 Anti-Laforin [N84/37.1R] Laforin Human Mouse IgG2a 114536 ...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Lu 3rd 10 8,205 Bassik Human CRISPR Knockout Library 101926 — 101934 Knockout Human Bassik...103,269 Human CRISPR Knockout Library 1000000132 Knockout Human X.S. Liu 3rd 10 185,634 Human Epigenetic Knockout...plasmid) Knockout Human Zhang 3rd 6 123,411 Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406...,406 Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...,658 Human Genome-Wide Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole...Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ... pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458...AHR_1 PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458..._BCL6_iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458...PX458_RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1...iso2 PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA...PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458...PX458_TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146...

  4. Resolute Plasmid Collection

    Type
    Collection
    ...Description Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...proteins. Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins...Inducible barcoded overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 ...) are the largest family of transporters in the human genome with approximately 450 identified members...collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221... knockout library targeting mouse SLC proteins. Human SLC Overexpression Library 213694 Superti-Furga ...Superti-Furga CRISPR knockout library targeting human transmembrane transporters. Feedback Please send...

  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find ...passaging of human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell (hiPSC) lines. The...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description...Institute for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS...

  6. CRISPR References and Information

    Type
    Collection
    ...elegans ), fruit fly, zebrafish, mouse, rat, and human. Developed by the Stanley Qi Lab . CCTop - CRISPR...location, and genome annotation. Available genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303... a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm...on-target activity for selected target(s). Uses human, mouse, and rat reference genomes. Hosted by the Broad...meningitidis Cas9 PAMs. Currently supports: mouse and human. Developed by the George Church Lab . CRISPR Optimal... GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP/enChIP to...GABPA, and RAD21 PDF, 138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...previously observed instance of human serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library...from the synthetic human phosphoproteome. The iSPI resource subdivides the 110,139 human phosphoserine site...V70) (DOCX, 551 KB) Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides...phosphosite library can then be used to identify candidate human substrates of kinases of interest by mass spectrometry...for iSPI, rEcoli XPS, pSerOTS-C1*). The synthetic human phosphoproteome. The Rinehart lab synthesized 110,139...: A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Gassaway...Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun JW, Lou...

  8. TALEN Engineering

    Type
    Collection
    ... ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011... ; Reyon and Tsai et al., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat ...Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed...Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional...REAL-Fast and FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website...

  9. Rett Syndrome

    Type
    Collection
    ...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link opens in a new window...Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (..., suggesting that restoring MECP2 expression in humans could be curative. (Link opens in a new window)...common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats are available from (Link opens...conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells ...mammalian or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single...This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected...(Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via...GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing genome-wide knockout screens...smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene...These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN...Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly... for deletion mutagenesis and gene targeting in human and mouse pluripotent stem cells. 48706 pTAL7b Joung... TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043...in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids...Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove...Engineering Reagents Assembly via serial ligation. Validated in zebrafish somatic cells. Keith Joung Zhang ...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7...DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . 339(6121)...

  13. p53 Pathway

    Type
    Collection
    ...p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations...mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere with .... Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury...45-53. PubMed PMID: 11780111 . p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B,...activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated...component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2...

  14. Brain Initiative Collection

    Type
    Collection
    ...Initiative aims to revolutionize our knowledge of the human brain through the development and application of...cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712...pAAV-CAG-DIO-ChR2(H134R)-eYFP Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic...pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct...core has 100% seq conservation between mouse and human Jonathan Ting 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2...

  15. Antibody Plasmid Collection

    Type
    Collection
    ...display vector system for producing human antibodies Synthetic Human Antibodies: Selecting and Evolving...Beavil Cloning and mammalian expression of mouse and human IgG monoclonals with reduced immune effector functions...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...collection - monoclonal antibodies extensively validated for neuroscience research applications from the...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ... stalwart research model. With more than 70% of human disease genes having an ortholog in zebrafish, this...has more recently emerged as a model system for human disease and translational research, including the...cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies, and ...Model Organism Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish...CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...expression atlas - A zebrafish-specific gene expression database that provides information about when genes are...active in developing embryos. zfRegeneration - A database for gene expression profiling during regeneration...

  17. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use...Gene Guide RNA expression plasmids for endogenous human genes Guide RNA expression plasmids for endogenous...described gRNA and Cas9 expression vectors and validated the use of RNAs produced by these vectors for ...

  18. Adenovirus Plasmids

    Type
    Collection
    ... to cause mild to severe respiratory disease in humans. Advantages of adenoviruses in research include...Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be...plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have inserts...for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5...

  19. CRISPR Guide

    Type
    Collection
    ...) limiting. This PAM is abundant throughout the human genome, but an NGG sequence may not be positioned...activate or repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors...CRISPRoff maintains gene silencing across much of the human genome following transient expression of the dCas9... mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology , 31 (9), 822–826. PMID...Norville, J. E., & Church, G. M. (2013). RNA-Guided Human Genome Engineering via CAS9. Science , 339 (6121...Yamamoto, T. (2014). Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system...Doudna, J. A. (2014). Enhanced homology-directed human genome engineering by controlled timing of CRISPR...

  20. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...endogenously-tagged gene variants or to differentiate human iPSCs into other cell types, as noted in the purpose...cellular background. All cell lines and phenotypic datasets that result from this effort will be broadly shared...

  21. Cancer Research Plasmids and Resources

    Type
    Collection
    ...cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor suppressor... National Cancer Institute (NCI) NCI Biomarker Database International Cancer Genome Consortium...

  22. Serotype Testing AAV

    Type
    Collection
    ...Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information about each catalog...option is an economical choice that can be used to validate the performance of various serotypes before conducting...

  23. Distribution to Industry

    Type
    Collection
    ...CRISPR/Cas9-mediated saturation genome editing of human TP53 exon 5–8. Phagemid Synuclein VHH Immune Library...Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to find plasmids ...comprehensive end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden...for research in Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...

  24. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...my target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary...nucleotide to the start of my target sequence? The human U6 promoter prefers a 'G' at the transcription start...IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could use 1ul of..., Science 2013 ) do you have any suggestions? To date, I have never used the Ex Taq. In our hands, Herculase...

  25. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag)...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 51486 pET21a-alpha-synuclein...Parkinson's Michael J Fox Foundation MJFF 194247 pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA SNCA GFP CAG... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary diffuse leukoencephalopathy...

  26. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...optimized PE2 enzyme containing RT optimized for human codons, additional nuclear localization signals,...protection added to 3’ tail of pegRNA to prevent RNA degradation For more in-depth information on these PE tools...

  27. TALEN Guide

    Type
    Collection
    ...investigating gene therapy options for a rare disease in humans, TAL effectors could become a key tool in your ... TAL effector targets. The site, which will be updated soon with more features, is already receiving hundreds...

  28. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-...Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I...Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-...

  29. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...functionally distinct interneurons in mice, non-human primates and humans. Nat Neurosci. 2020 Dec;23(12):1629-1636...action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat Commun. 2018 Aug...pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. PLoS...Caveolae respond to acute oxidative stress through membrane lipid peroxidation, cytosolic release of CAVIN1...cellular compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal...Cytosolic or mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed...targeted DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...

  30. Neurodegeneration Research Collection

    Type
    Collection
    ...astrocytes. Endo et al Science. 2022 Nov 4. Base edit human mitochondrial DNA using mitoBEs . Yi et al. Nat ...new window) Michael J Fox Foundation (Link opens in a new window) A foundation dedicated to finding a cure...Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource page ...will advance more quickly with the avid sharing of data, tools, resources, methods, and results that are...living with Parkinson's today. The Michael J. Fox Foundation has made a number of tools (Link opens in a new...page . CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics... the progression of Huntington’s disease. The foundation offers curated information on tools and reagents...

  31. COVID-19 Resources

    Type
    Collection
    ...genes, see the links and references below: ACE2 - (Human Angiotensin Converting Enzyme 2) is the host cell...Scientists have been working at lightning speed to elucidate as much as they can about this novel virus. It...lab has utilized Cas12a’s non-specific ssDNA degradation to develop the method termed DNA Endonuclease...COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI/PubMed) Protocols Protocols...

  32. Caltech Systemic Capsids

    Type
    Collection
    ...adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech...

  33. Luciferase Plasmid Collection

    Type
    Collection
    ... CIneo-RL Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18... animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission of photons...

  34. Antibody Guide

    Type
    Collection
    ...section below. The following table describes the five human isotypes. Some isotypes naturally form multi-antibody... of interest that are validated for your assay type. See the Antibody Validation section below for more...sourced from the same species. Antibody Validation Validating an antibody ensures that the antibody specifically...the assay type you are running. Sufficient validation data specific to your antibody and assay may already...you’ll need to validate the antibody upon receiving it. If an antibody fails validation, select a new ...process through sequential rounds of validation. Validation of an antibody is a process separate from running...Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate the identity...

  35. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Plasmid Kits and Collections Human Kinase ORF Kit : 558 distinct human kinases and kinase-related protein...expression. Myristoylated Kinase Kit : Consists of 190 human kinases and kinase-related open reading frames (...assay data for translational and clinical research. Sci Data. Feb 27;5:180015. doi: 10.1038/sdata.2018.15...TNFSF7 CECR1 cat eye syndrome chromosome region, candidate 1 ADGF, IDGFL CER1 cerberus 1, cysteine knot superfamily..., mu 1 KIAA0403, LMOR, MOR, MOR1, OPRM OSGIN1 oxidative stress induced growth inhibitor 1 BDGI, OKL38 ...library from Anne Messer and the Michael J Fox Foundation is a synuclein phagemid VHH phage display library...
Showing: 1 - 35 of 35 results