...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR systems, including...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...the entire population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) ...

...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...Mouse line with conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced ...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link....806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold A140V...A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU 808delC Arg270Glufs...Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold BO_DI 806delG G269Afs...G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts...

...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite...model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39. PMID: 26310623... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite....9 When using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon... using the AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...

... has published information and protocols for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus...Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR... reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV...Qi's lab. COVID-19 Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase...Starr). External Resources Research Articles Many publishers have allowed free access to articles related ...of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research Registry...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

... ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID ...Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...note that the following table lists both published and unpublished plasmids containing gRNA sequences designed...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...prepare and compile resources for this meeting's publication, we are asking participants to share practical...have final approval before anything is released publicly, but please only share materials that you are ...materials that you have permission to share (e.g., publication's permission). Connie Cepko, Harvard Medical School...section for how to best help contribute to this publication. Citation information for openly shared communication...

...CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview...Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into... before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...

... the original publication below. You can also download the full-text of the publication here . Article... of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs details...7 - 14 days. Vary these times depending on the doubling time of the cell line used. NOTE: This incubation...10.3791/52118 (2015). PubMed For the complete publication, including representative results and discussion...

...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty Prime...

...from the Zhang group . Many protocols have been published with thorough step-wise instructions on designing...references. For genome editing, the Zhang lab has published this Nature Protocol article . Should I add the...very robust in this case for EMX1. Since the publication of our paper, we have two new optimized primers...primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...