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We narrowed to 154 results for: TOR;

Showing: 81 - 120 of 154 results

  1. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation Depositor...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ... Depositor Collections Botman-Teusink Yeast FP Collection...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for engineering...

  3. Recombinases AAV Preps

    Type
    Collection
    ...plasmids in the repository. Browse our AAV collection of site specific recombinases.... Viral Vector Packaging ...Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... Depositor Collections New England Biolabs Cell-Imaging Plasmid...Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression...expression under T7 control 101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian... cell surface localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient...for cell surface localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian...mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian...

  5. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger...tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc...aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc...OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...

  6. Distribution to Industry

    Type
    Collection
    ...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...also adding additional filters such as expression, vector type, species, and more. Plasmids Browse the table...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

  7. Worm Expression Resources

    Type
    Collection
    ...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

  8. Arf GTPase Family

    Type
    Collection
    ...References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized...exchange factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles)...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

  9. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGATGGCGAGAACAGGGcggtggagtataacgcTGTGGGCCTGACGAAGGA Complement Factor H TAL3230 & TAL3231 TGATTACATGTGAACCTCcatcacgtgaccagcatgTGTCCACCTCCTTTTGGA...TAAACGCCCTGATCTTCAgcgccgacggcctgcagcAGAGAGACAAGATGCGCA inhibitor of DNA binding 3 TAL3466 & TAL3467 TCAACATTGGATATCTATagagctttacagctagTTTATCCCAGTGTCCAGA...TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA Purinergic receptor Y2 TAL3348 & TAL3349 TTCAACGCATCAGTGAATgttcaaaaccacagttCTGCACACAGCTGCAGCA...TCTTTTTGTGCTGCGCCAcccagcccataatcgaGTCTTCTACAGGCATGAA retinitis pigmentosa GTPase regulator b TAL3528 & TAL3529 TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA...TGGAGAAACAGAAGATGAaatccccgagtccggaGCTGTCTTCACTTTTGGA retinitis pigmentosa GTPase regulator b ORF15 TAL3530 & TAL3531 TAAGAGGGCGGAGCTTTTatctcgcaaagctctgCCCACTGAGTTACTTAGA...TGACTGGGAGGAAGATCAgagtcccgttgtgtctTGCAGCTCAGGATTTGGA Vascular-endothelial growth-factor-aa (vegfaa) TAL3200 & TAL3201 TCTCGCTTTGCTTCCTCCtcagtgattcccttcaTGGATGTGTATAAAAAGA...

  11. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

  12. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Plasmid Gene/Insert Vector Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...expression plasmid based on factors such as selectable marker or cloning method. When using CRISPR, you...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  13. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

  14. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties... into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

  15. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids...Pseudotyping Want to save time? Check out our Viral Vector Packaging Service and learn about our options for...RepCap Plasmids for Serotypes Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid ...species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have suggestions...

  16. Open Enzyme Collection

    Type
    Collection
    ... Depositor Collections FreeGenes Project Open Enzyme Collection...Enzymes You may also like… Bacterial Expression Vectors Synthetic Biology Plasmids BIOFAB Plasmid Set Drew... own enzymes by cloning them into an expression vector of their choice and transforming into E. coli bacteria...Researchers should clone these enzymes into an expression vector of their choice and then transform into E. coli...expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all ...

  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Protein Collection . Return to top Secretory Pathway The secretory pathway is named for its role in secreting...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting... 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin...

  18. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  19. Brain Armamentarium

    Type
    Collection
    ...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru 163509-PHPeB CN1839...CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for...BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain...

  20. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... Depositor Collections Rinehart Lab Phosphoprotein Reagents...Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated... via partial UAG codon reassignment and release factor 1 deletion . FEBS Letters , 586 (20), 3716–3722...

  21. Cell Migration Consortium Plasmids

    Type
    Collection
    ... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

  22. Impact of Genomic Variation on Function (IGVF) Consortium Collection

    Type
    Collection
    ... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

  23. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  24. Fluorescent Protein Guide

    Type
    Collection
    ...is a Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular...signals, cells, or groups of cells with either actuators or sensors. Chemogenetics Plasmids | Guide Use...molecules to activate genetically engineered cellular receptors that affect signalling pathways within cells. ...

  25. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... and type V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that...target site. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB...

  26. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

  27. Antibody Production

    Type
    Collection
    ...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

  28. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )... ( e.g., with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells ...

  29. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Modular Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

  30. Antibodies

    Type
    Collection
    ...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

  31. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...catalytically dead Cas13b creates a programmable RNA editor that converts adenosine to inosine in RNA. Since...effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain...

  32. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase... a specific locus, avoiding known transcription factor and other protein binding sites. Browse, sort, ...

  33. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...gRNA expression vectors available from Addgene....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...

  34. Adenovirus Plasmids

    Type
    Collection
    ...Collection of adenovirus shuttle vectors and strains...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

  35. Lentivirus Plasmids

    Type
    Collection
    ...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619 pLB 3rd Modification of...promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730 pLionII 3rd FIV-derived...

  36. The Pleiades Promoter Project

    Type
    Collection
    ...Project - A regulatory toolbox of MiniPromoters to drive selective expression in the brain.... Depositor Collections...., Wasserman, W. W., Simpson, E. M. (2010). A regulatory toolbox of MiniPromoters to drive selective expression...

  37. Genetic Code Expansion

    Type
    Collection
    ...sites changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306...tolC, and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead...amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...

  39. Synthetic Biology - Algal

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...

  40. Synthetic Biology - Bacterial

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do ...
Showing: 81 - 120 of 154 results