We narrowed to 101 results for: Cre

...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both types of base editors...adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor... to the PAM sequence, some base editing systems create a wide spectrum of single-nucleotide variants (...

... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics Institute...), including plasmids for protein production, secretion, and codon-optimized versions designed...metalloprotease that cleaves hACE2, potentially increasing viral infectivity (Heurich et al., 2014). PCP4...-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations... Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike...Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice, Ts1Rhr . International ...

...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. Most antibodies...functional neural circuits at the growth cone. Image credit: IPI Browse Netrin, DCC, and IGDCC3 (PUNC) Antibodies...postsynaptic membranes at neuronal synapses. Image credit: IPI Browse Neurexin and Neuroligin Antibodies ...function, and/or detect integrin expression. Image credit: IPI Browse Integrin Antibodies ID Recombinant ... Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...

...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...smaller fragment of an antibody, which could offer increased stability and ease of production due to their ...highlights plasmid collections that can be used to create and express plasmid-based antibodies. Description...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...

...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...for cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors... our website. Placing an Order: After you have created an account, you can place orders through our website...

...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function... et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...community. This collection highlights plasmids created with support from the BRAIN Initiative. Jackson...Link opens in a new window) . This resource was created with support from the Chan Zuckerberg Initiative...

...Mammalian Expression HcRed1 588 618 0.3 Dimer pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression...plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest. Plasmid...Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22 5.5 Dimer dKatushka-pBAD - Bacterial Expression...community-editable database for fluorescent proteins created by Talley Lambert Content last reviewed: 25 September...

...carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and maintaining... accelerate scientific discovery that will help create new and more effective therapies for these tumors...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

... plasmid kits and collections are designed for creating plant expression vectors. ID Kit Description Cloning... binary vectors — High-performance vectors for creation of fusion constructs in transgenic analysis of... in just two simple cloning steps. CRISPR Kate Creasey Krainer , Vladimir Nekrasov , Nicola Patron Additional... to understand how scientific publications are created. Papers from the plant biology literature are discussed...

...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...

... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene...mTORC1 promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References...

...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants...

...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...

...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute...

...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com. Browse, sort, or search the...

... modifiers include: P300 — activation through increased H3K27 acetylation TET1 — activation through cytosine...Overview of epigenetic modification using CRISPR. Created with BioRender.com. Browse, sort, or search the...

...breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...

...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...