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Addgene
Showing: 41 - 60 of 71 results

  1. Chemogenetics Plasmids

    Type
    Collection
    ...on the ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are ...

  3. Resolute Plasmid Collection

    Type
    Collection
    ...and pharmaceutical companies. The RESOLUTE project has received funding from the Innovative Medicines Initiative...

  4. CRISPR References and Information

    Type
    Collection
    ...finger nuclease sites, this tool from the Joung Lab has been expanded to identify potential DNA target sites...cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall and Matthews CRISPR/Cas9...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion proteins...protein of interest with a fluorescent protein that has a different emission wavelength. For example, if ...mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU GFP Vamsi Mootha 49153 GFP-Mff Mitochondria-Outer...

  6. AAV for Neuronal Tracing

    Type
    Collection
    ... delivery by an AAV. Since this starter cell now has all the viral genes present, the virus can spread...

  8. Retrovirus Plasmids

    Type
    Collection
    ... expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated...Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor...

  10. Antibody Production

    Type
    Collection
    ...in one of its recommended assays. If an antibody has passed stability testing, it may ship at room temperature...

  11. Microbiology Resources

    Type
    Collection
    .... Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms...

  12. Worm Expression Resources

    Type
    Collection
    ...Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory and described...

  14. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Express gene of interest from the CMV promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro...

  15. AAV Packaged on Request

    Type
    Collection
    ...range of experiments, both in vitro and in vivo. It has low immunogenicity, is easy to use, and can be safely...

  16. Brain Initiative Collection

    Type
    Collection
    ...support from the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...

  17. Genetic Code Expansion

    Type
    Collection
    ...its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids...aminoacylate the orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

  18. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...molecules in either of these types of studies Addgene has a collection of plasmids suitable for creating individual...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ...development. Unlike other cell types, each stem cell has the potential to either remain a stem cell or become... concerns related to embryonic stem cell use and has the major advantage that iPSCs can potentially be...

  20. Brain Armamentarium

    Type
    Collection
    ...forebrain GABAergic interneurons. The enhancer core has 100% seq conservation between mouse and human Jonathan...
Showing: 41 - 60 of 71 results