We narrowed to 45 results for: crispr grnas

...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires... of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Lentiviral Preps CRISPR Protocols...of empty gRNA vectors. Explore our most popular plasmids for expression of gRNA and Cas, gRNA only, or... Multiplex gRNA Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA plasmids, including...MoClo CRISPR/Cas Toolkit for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids...Empty gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR... CRISPR Empty gRNA Vectors...

...Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips... curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community... CRISPR References and Information CRISPR References and Information Addgene Resources CRISPR Software...inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel... Listed - a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting...Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA design...

...used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your...testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs is particularly...plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering...Design and Clone gRNA Select Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... about visualization using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Visualize CRISPR Plasmids:...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...

... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was originally employed...generating gRNAs makes CRISPR one of the most scalable genome editing technologies, making CRISPR perfect...optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA design...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL... CRISPR Protein Tagging CRISPR Plasmids...Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Parasites CRISPR Plasmids... Addgene's parasite CRISPR plasmid collection also includes the Toxoplasma CRISPR Knockout Pooled Library...protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...below for CRISPR activation plasmids. To learn more about CRISPR activation and other CRISPR topics, read...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...more about purification using CRISPR and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid... CRISPR Purify CRISPR: Purify Genomic ...genomic DNA bound by the gRNA. An epitope tag or tags can be fused to dCas9 or gRNA for efficient purification... using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Xenopus CRISPR Plasmids: Xenopus Cut...The following CRISPR plasmids have been designed for use in Xenopus. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in Xenopus. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...The following CRISPR plasmids have been designed for use in zebrafish. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR C. elegans CRISPR Plasmids: C. ...The following CRISPR plasmids have been designed for use in C. elegans. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in C. elegans. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources...below for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics, read...Return to top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...following CRISPR plasmids have been designed for use in Drosophila and other insects. To find emtpy gRNA plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... CRISPR Drosophila CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Plants CRISPR Plasmids: ... The following CRISPR plasmids have been designed for use in plants. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR expression plasmids that have been designed for use in plants. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Yeast CRISPR Plasmids: Yeast Activate... The following CRISPR plasmids have been designed for use in yeast. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in yeast. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols... CRISPR Bacteria CRISPR Plasmids: Bacteria...The following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids, visit...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in bacteria. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...tables below for CRISPR nickase plasmids. To learn more about CRISPR nickases and other CRISPR topics, read... CRISPR Nick CRISPR Plasmids: Nick...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...

...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...following CRISPR plasmids have been designed for use in mammalian expression systems. To find emtpy gRNA plasmids...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids ...Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...