...Genome Engineering: Basic Principles Genome Engineering: Existing Technologies Get Genome Engineering tools... Genome Engineering Videos CRISPR Technology and Applications CRISPR-Cas...performed by Patrick Hsu, David Scott, and others in the Feng Zhang Lab. The CRISPR-based transcriptional modulation...

...1504. Xiaodong Liu Calcium NEMO calcium sensors Engineering of NEMO as calcium indicators with large dynamics...indicator for two-photon imaging at fiber laser wavelengths. Nat Methods. 2020 May 25. pii: 10.1038/s41592...dynamics of ATP in single cells. Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ...neurons, astrocytes) A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface...insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Sci Signal...measurement Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science...Nanosensors for in vivo measurements of citrate (CitA) Engineering genetically encoded nanosensors for real-time...

...pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-5-PV2509 29777...pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-8-PV0142 105534-...ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-1-PV0102 105531-AAV1 pAAV.CMV.LacZ.bGH James ...ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH...ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH...

... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD...Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440...

...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

...Background Chemogenetics plasmids encode genetically engineered receptors that are activated by specific small...control signaling within cells expressing these engineered receptors. Chemogenetic receptors are popular...behavior. Depending on the signalling pathway the engineered receptor is coupled to and the specific cell ...are G-coupled protein receptors that have been engineered to respond to specific physiologically inert ...while hMD4(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains...

...light-inducible site-specific iCreV recombinase Hongkui Zeng 140135-PHPeB pAAV-EF1a-iCreV can be used to generate...light-inducible site-specific iCreV recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the...

...before starting your experiment. Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc...Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries... Cell Migration Consortium Center for Genome Engineering EXtracellular Plasmid RESource (EXPRESs) Consortium...

...reproducibility. Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed... functions Expression of IgG Monoclonals with Engineered Immune Effector Functions. Methods Mol Biol. ...6:46. John McCafferty CRISPR system to rapidly engineer the constant immunoglobulin domains to obtain ...hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822. Scheeren...

...to CRISPR technology Introductory video from the Feng Zhang lab Addgene Blog Posts How to Design Your ...silkworm, and budding and fission yeast. Target Finder (Feng Zhang lab) Identifies gRNA target sequences from... non-model invertebrate species. Forums and FAQs Feng Zhang lab: Tips for CRISPR design, homology directed... cloning . CRISPR Discussion Group (moderated by Feng Zhang lab): Forum/FAQ Drosophila: General Advice...

...Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Zeng 51669 AAV...pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP Syn EGFP Control Roth 50469...phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 183412 pAAV-CAG-FlpO CAG Flpo expression Recombinases...

...be found on the TALengineering website. Users may also wish to join the TALengineering newsgroup . ID ... TALengineering Reagents TALE Repeat Plasmids Individual REAL...

... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...Users are also encouraged to join the Genome Engineering newsgroup here . Links to additional pages describing...Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM specificities...

...metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and proteomic ...voltage all contribute to assess ligand action/engagement. Protein production is used for assays and to...

...Luciferases AkaLuc : A modified firefly luciferase engineered to use the red-shifted substrate Akalumine with...bioluminescence imaging in vivo . Luciferases with tunable wavelengths : NanoLuc® with either SNAP‐tag or HaloTag7 tag...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly...Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase Renilla...

... TALengineering Reagents TALEN Reagents for Human Genes Engineered TALENs for Endogenous...high-throughput FLASH method to construct a large series of engineered TALENs targeted to human genes involved in cancer...

... TALengineering Reagents TALEN Reagents for EGFP Engineered TALENs for the EGFP Reporter...high-throughput FLASH method to construct a large series of engineered TALENs targeted to 48 sites in the EGFP reporter...

...GTPases and their GEFs and GAPs: concepts and challenges Sztul E, Chen PW, Casanova JE, Cherfils J, Dacks...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GTPase Arf1 375...available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems GAP Arfgap1 55738...

...dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic worm lines. The transparent...below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids... nematodes. Wormbuilder - Describes methods to engineer the genome of the genetic model organism C. elegans...

...into a single construct would be technically challenging. Thanks to efforts by the Bogdanove group and...targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...gene disruption in somatic zebrafish cells using engineered TALENs. Sander JD, Cade L, Khayter C, Reyon D...