... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

... Genome Engineering Although overexpressing or knocking down a gene can tell you much about its function.... Return to Top Protein Purification Sometimes looking at a protein inside cells just isn’t enough to ...Growth of strain USO hisB- pyrF- rpoZ- on plates lacking histidine and containing 3-AT Scot Wolfe Bacterial...- pyrF- rpoZ- , this promotes growth on plates lacking His and containing 3-AT. FLIParaF.Ec-250n 18855...

... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...Foundation Neurodegeneration Research Collection Parkinson's Disease Plasmid Resource Rett Syndrome Plasmid...

... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...

...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

...Baylor College of Medicine Gene Vector Core have kindly deposited a series of lentiviral and adenoviral... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors ...

...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short... flank the MCS for improved normalization. John Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3'...

...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...Cre inhibition", "Boyden 2014", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ... Truncated SARS-CoV-2 Spike gene constructs for making VSV pseudovirus Common human coronavirus (OC43,...

...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Recommended Applications PI Don’t See What You’re Looking For? Please let us know what you would like to ...

...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

... Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...protocol videos available that may be helpful for working with this plasmid collection. Single cell passaging...