Viral Service: PHP.S AAV
As part of our Viral Service, Addgene is producing ready-to-use viral preparations in the PHP.S serotype from the Viviana Gradinaru laboratory. These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006).
The PHP.S serotype exhibits efficient transduction of the peripheral nervous system via systemic delivery in adult animals. In vivo studies showed that, relative to parent capsid AAV9, intravenous injection of PHP.S AAV led to an increase in both the number of transduced cells and the expression level per cell. PHP.S AAV was also shown to transduce neurons in the enteric nervous system and in other peripheral ganglia, such as the cardiac ganglia. When used with Cre transgenic lines or cell-type-specific promoters, this serotype could enable targeted transduction of subsets of cells within these regions.
As part of our standard viral production, these viral vectors undergo quality control, including AAV titering, confirmation of the transfer plasmid with PCR, purity assessment by SYPRO Red staining, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.
Browse Available PHP.S AAV
When using the PHP.S serotype in future publications, please acknowledge Viviana Gradinaru and Benjamin Deverman and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.
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