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Viral Service: Caltech Systemic Capsids


As part of our Viral Service, Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center. As part of our standard viral production, these viral vectors undergo quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector preparation.

Systemic Delivery AAV Capsid Choice

Need help deciding which capsid to choose? The following decision tree was developed at the CLOVER Center at Caltech and can help guide your decision.

AAV decision tree. Described under the heading Text Description for the AAV Decision Tree
Decision tree for choosing your AAV capsid.

Your choice could depend on several factors including: the delivery method, mouse strain, target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang, et al., 2019) for LY6A-permissive mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center and was adapted from Challis et al. (2022).
Text version of the AAV decision tree can be found at the bottom of this page.

For more in depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids. To review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use.

PHP.eB

These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005).

Note on PHP.eB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (DOI 538421) to cross the blood brain barrier. For information on whether the PHP.eB serotype will work in your mouse line, see Supplementary Table 3.

Browse Available PHP.eB AAV

ID Name Promoter Description Category PI
Controls
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
37825 pAAV-CAG-GFP CAG GFP Control Boyden
50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden
83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell
104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru
104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru
105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson
105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson
114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth
117382 hSyn1-eYFP Syn EYFP Control Gradinaru
135630 pAAV-S5E2-dTom-nlsdTom E2 regulatory element dTomato Control Dimidschstein
135631 pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein
163505 CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA minBetaGlobin SYFP2 Control Ting
163509 CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA hSyn1 H2B-SYFP2 Control Ting
Chemogenetics
44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth
44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth
135635 pAAV-S5E2-Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein
Biosensors
104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim, GENIE
104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim, GENIE
104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim, GENIE
104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim, GENIE
104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim, GENIE
104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim, GENIE
135632 pAAV-S5E2-GCaMP6f E2 regulatory element Calcium sensor GCaMP Dimidschstein
100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor RGECO Kim, GENIE
Optogenetics
26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator ChR2 Deisseroth
20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent ChR2 Deisseroth
127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent ChR2 Gradinaru
135633 pAAV-S5E2-C1V1-eYFP E2 regulatory element Activator C1V1 Dimidschstein
135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein
Recombinases
105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson
105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson
140135 pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng
140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng
140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng
164450 CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA minBetaGlobin iCre expression Cre Ting

PHP.S

These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006).

Browse Available PHP.S AAV

ID Name Promoter Description Category PI
28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden
59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden

PHP.V1

These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847).

Browse Available PHP.V1 AAV

ID Name Promoter Description Category PI
104052 pAAV-CAG-DIO-EYFP CAG EYFP, Cre-dependent Control Gradinaru

MaCPNS1

These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136).

Browse Available MaCPNS1 AAV

ID Name Promoter Description Category PI
37825 pAAV-CAG-GFP CAG GFP Control Boyden

MaCPNS2

These viral vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137).

Browse Available MaCPNS2 AAV

ID Name Promoter Description Category PI
37825 pAAV-CAG-GFP CAG GFP Control Boyden

CAP-B10

These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004).

Browse Available CAP-B10 AAV

ID Name Promoter Description Category PI
37825 pAAV-CAG-GFP CAG GFP Control Boyden

CAP-B22

These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005).

Browse Available CAP-B22 AAV

ID Name Promoter Description Category PI
37825 pAAV-CAG-GFP CAG GFP Control Boyden

Citation Information

When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows:

Don’t See What You’re Looking For?

Please let us know what you would like to see available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would be helpful to the scientific community.

Text Description for the AAV Decision Tree

The AAV decision tree graphic above helps you choose the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species, and then cell type.

For CNS:

  • For infant rhesus macaques:
    • Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2
    • Cell type-biased:
      • Neurons - AAV.CAP-Mac, AAV9-X1.1
  • For marmosets:
    • Broad tropism - AAV.CAP-B22
    • Cell type-biased:
      • Neurons - AAV.CAP-B10
      • Brain vascular cells - AAV.CAP-Mac
  • For rats:
    • Broad tropism - AAV-PHP.eB
    • Cell type-biased:
      • Neurons - AAV.CAP-Mac
      • Brain vascular cells - AAV9-X1.1, AAV1-X1
  • For mice:
    • Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC
    • Cell type-biased:
      • Astrocytes - AAV-PHP.AX with GRE or transgenic animal
      • Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal
      • Brain vascular cells - AAV9-X1.1, AAV1-X1
      • Neurons - AAV.CAP-B10, AAV-PHP.N
        • Broad - AAV-PHP.AX with GRE or transgenic animal
        • L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal
        • L2/3 & L4 - AAV.CAP-B10 with GRE or transgenic animal
        • Excitatory - AAV.PHP.N with GRE or transgenic animal

For PNS applications in rodents and NHPs:

  • Broad tropism - AAV-MaCPNS2, AAV-PHP.S
  • Sensory nervous system - AAV-MaCPNS1

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