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173,926 results

Showing: 61 - 80 of 173926 results

  6. pcDNA3.1 mycBioID

    Plasmid
    #35700
    Purpose
    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    cmv
    Available Since
    April 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    35700-plasmid-map-sequence-id-228311

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  8. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  16. pAAV2/8

    Plasmid
    #112864
    Purpose
    AAV packaging plasmid expressing Rep/Cap genes
    Depositor
    James M. Wilson
    Insert
    Rep2/Cap8
    Use
    AAV
    Promoter
    Truncated P5
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112864-plasmid-map-sequence-id-333531

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  19. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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Showing: 61 - 80 of 173926 results