165,688 results

1. pcDNA3-EGFP

   Plasmid

   #13031

   Purpose

   Mammalian expression vector for cloning your gene fused to EGFP

   Depositor

   Doug Golenbock

   Insert

   Enhanced Green Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-dCas9-VPR

   Plasmid

   #63798

   Purpose

   SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   SP-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1 - TRC cloning vector

   Plasmid

   #10878

   Purpose

   3rd generation transfer plasmid

   Depositor

   David Root

   Article

   Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 Basic Vector

   Plasmid

   #212936

   Purpose

   Firefly luciferase vector for investigating regions controlling transcription

   Depositor

   Debrya Groskreutz

   Type

   Empty backbone

   Use

   Luciferase

   Mutation

   WT

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLV-mitoGFP

   Plasmid

   #44385

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoGFP (COX8A Human)
   

   Use

   Lentiviral

   Tags

   Cox8 targeting sequence and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti dCAS-VP64_Blast

   Plasmid

   #61425

   Purpose

   3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Lentiviral Prep

   Insert

   dCAS9(D10A, N863A)-VP64_2A_Blast
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HsPeg10rc3

   Plasmid

   #174859

   Purpose

   Expresses recoded human Peg10 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   recoded Homo sapiens PEG10 (PEG10 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXR001: EF1a-CasRx-2A-EGFP

   Plasmid

   #109049

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Insert

   CasRx
   

   Use

   CRISPR and Lentiviral

   Tags

   2A-eGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Hs.cargo(Cas9)

    Plasmid

    #174864

    Purpose

    Expresses human Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by HsPeg10 UTRs
    

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-VSV-G

    Plasmid

    #8454

    Purpose

    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. OpenCRISPR-1

    Plasmid

    #221565

    Purpose

    Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    tTRE, hPGK

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Tobias Meyer, Sabrina Spencer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-CreEGFP

    Plasmid

    #165140

    Purpose

    Expression of Cre recombinase fused with EGFP in C-terminal

    Depositor

    Daekee Lee, David Threadgill

    Insert

    Cre recombinase
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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