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pLminP_Luc2P_RE57

Plasmid

#90402

Purpose

Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ISRE

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2

Plasmid

#52961

Purpose

Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

Depositor

Feng Zhang

Article

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only

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pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Blast

Plasmid

#83480

Purpose

Mammalian expression of Cas9 and sgRNA scaffold

Depositor

Mohan Babu

Insert

Blasticidin S deaminase

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Replaced puromycin N-acetyltransferase on the ori…

Promoter

EF-1α

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_D210N

Plasmid

#111904

Purpose

Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Mutation

Changed D 210 to N

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-PR rep/cap

Plasmid

#197565

Purpose

encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

Depositor

Casey Maguire

Article

Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

Inserts

AAV Rep genes
AAV9 VP1 Cap gene with PR insert

Use

AAV

Mutation

contains 7mer insert PRPPSTH between amino acids …

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUCM-AAVS1-TO-hNGN2

Plasmid

#105840

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
mCherry
rtTA3G

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCBASceI

Plasmid

#26477

Purpose

I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

Depositor

Maria Jasin

Article

Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

Insert

pCBASceI

Tags

HA

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pEM040 [Multiome Perturb-seq sgRNA expression vector]

Plasmid

#224898

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

Depositor

Thomas Norman

Article

Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Cytoplasmic PancACe

Plasmid

#215708

Purpose

Expresses acetyl-CoA biosensor in the cytoplasm

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and cytoplasmic localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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ppyCAG_RNaseH1_WT

Plasmid

#111906

Purpose

Express RNASEH1 in mammalian cell.

Depositor

Xiang-Dong Fu

Article

Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

Insert

RNASEH1 (RNASEH1 Human)

Tags

V5

Expression

Mammalian

Available Since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pZT-C13-R1

Plasmid

#62197

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Right CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro

Plasmid

#248161

Purpose

Expresses KRAB fused to dCas9 and BFP upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

KRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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Mitochondrial PancACe

Plasmid

#215710

Purpose

Expresses acetyl-CoA biosensor in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSI-Check2-hRluc-NFkB-firefly

Plasmid

#106979

Purpose

Luciferase reporter with internal control for NFkB signal.

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

NFKB recognition sites (NFKB1 Human)

Use

Luciferase

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pZT-C13-L1

Plasmid

#62196

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Left CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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