166,778 results

1. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SV40 and minCMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. eeBxb1

   Plasmid

   #222339

   Purpose

   Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Promoter

   EF1a

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/9n

   Plasmid

   #112865

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap9
   

   Use

   AAV

   Promoter

   Truncated P5

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-TurboID-NES_pCDNA3

   Plasmid

   #107169

   Purpose

   expresses V5-tagged TurboID in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Hygro

   Plasmid

   #127763

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmScarlet3_C1

   Plasmid

   #189753

   Purpose

   C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRGEB32

    Plasmid

    #63142

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBR1MCS-2

    Plasmid

    #85168

    Purpose

    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

    Depositor

    Kenneth Peterson

    Article

    Kovach et al Gene. 1995 Dec 1;166(1):175-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Constitutive (derepressed P-lac)

    Available Since

    Nov. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Tags

    mStayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. beta-arrestin1 mYFP

    Plasmid

    #36916

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    beta-arrestin1 (Arrb1 Rat)
    

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. VEGFR2-mCh

    Plasmid

    #108854

    Purpose

    Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

    Insert

    KDR (KDR Human)
    

    Tags

    Labeled with mCherry on the C-Terminus via a 3 am…

    Expression

    Mammalian

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-3-VEGFR3

    Plasmid

    #119230

    Purpose

    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

    Depositor

    Kari Alitalo

    Insert

    VEGFR3 (FLT4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-PDGFRB

    Plasmid

    #23893

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTFE3-donor

    Plasmid

    #112377

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

    Depositor

    Kevin White

    Insert

    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    

    Use

    CRISPR

    Mutation

    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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