166,289 results

1. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Expression

   Mammalian

   Mutation

   Isoform 2

   Available Since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-mCherry

   Plasmid

   #50459

   Purpose

   Double floxed mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Promoter

   human Synapsin 1

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-beta-3

   Plasmid

   #27289

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin beta 3 (ITGB3 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB80-hKif5b(1-560)rigor-2xmNeongreen

   Plasmid

   #174649

   Purpose

   Live-cell marker for stable microtubules

   Depositor

   Lukas Kapitein

   Article

   Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

   Insert

   hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
   

   Tags

   mNeongreen-mNeongreen

   Expression

   Mammalian

   Mutation

   rigor mutation G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ApoE4

   Plasmid

   #197148

   Purpose

   Expresses ApoE4 in Mammalian cell

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   ApoE4 (APOE Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIC28-Bsa4

   Plasmid

   #26103

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

   Plasmid

   #200016

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMDLg/pRRE

    Plasmid

    #12251

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    HIV-1 GAG/POL
    

    Use

    Lentiviral; Packaging

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. BFP-Rab5

    Plasmid

    #49147

    Purpose

    mammalian expression of BFP-Rab5 fusion

    Depositor

    Gia Voeltz

    Article

    Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

    Insert

    Rab5 (RAB5B Human)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT GBA pcDNA3.1

    Plasmid

    #188580

    Purpose

    Ectopic expression of wild-type GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV R-CEPIA3mt

    Plasmid

    #140464

    Purpose

    Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

    Insert

    R-CEPIA3mt
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ApoE2

    Plasmid

    #197146

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE2 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEN-luxCDABE

    Plasmid

    #44918

    Depositor

    Harry Mobley

    Article

    Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    sigma 70 promoter
    

    Use

    Luciferase

    Tags

    lux transcriptional fusion

    Promoter

    em7

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FAH-rtTA3G

    Plasmid

    #120309

    Purpose

    An AAV vector that expresses rtTA3G under the c-fos promoter

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    rtTA3G
    

    Use

    AAV

    Promoter

    c-fos

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST exorh:mCherry

    Plasmid

    #195989

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Nicolas Rohner

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-EXO1a

    Plasmid

    #111629

    Purpose

    Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

    Insert

    Exonuclease 1a (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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