Michael O'Donnell Lab: O'Donnell lab plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239197 | Sc PCNA-pET(11a) | Overexpress Sc PCNA in E. coli |
| 239198 | Sc Pol3-FLAG(Ec)-pRSF405/GAL | Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer) |
| 239199 | Sc [Pol31 + Pol32]-pRS403/GAL | Overerxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer) |
| 239200 | Sc RFC[hk-1s + 5]-pLANT/RIL | Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli |
| 239201 | Sc FLAG-RFC1-pRSFDuet | Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli |
| 239202 | Sc RFC5-pCDFDuet | Overexpress Sc RFC5 in E. coli |
| 239203 | Sc RFC[2+3+4]-pET | Overexpress Sc RFC2, RFC3, & RFC4 in E. coli |
| 239204 | Sc Rad27-pET24 | Overexpress Sc Rad27 (FEN1) in E. coli |
| 239205 | Sc Rad27-S6- pET24 | Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli |
| 239471 | Sc FLAG-S6-PCNA-pET | Overexpress Sc FLAG-S6-PCNA in E. coli |
| 239473 | Sc FLAG-S6-RFC1-pRS405/GAL-L | Overexpress Sc FLAG-S6-RFC1 in yeast (integrated) |
| 239474 | Sc RFC[2+5]-pRS403/GAL | Overexpress Sc RFC2 & RFC5 in yeast (integrated) |
| 239475 | Sc RFC[3+4]-pRS402/GAL | Overexpress Sc RFC3 & RFC4 in yeast (integrated) |
| 239476 | Sc FLAG-RFC1-pRS405/GAL-L | Overexpress Sc FLAG-RFC1 in yeast (integrated) |