Michael O'Donnell Lab: O'Donnell lab plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 239197 | Sc PCNA-pET(11a) | Overexpress Sc PCNA in E. coli |
| 239198 | Sc Pol3-FLAG(Ec)-pRSF405/GAL | Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer) |
| 239199 | Sc [Pol31 + Pol32]-pRS403/GAL | Overerxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer) |
| 239200 | Sc RFC[hk-1s + 5]-pLANT/RIL | Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli |
| 239201 | Sc FLAG-RFC1-pRSFDuet | Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli |
| 239202 | Sc RFC5-pCDFDuet | Overexpress Sc RFC5 in E. coli |
| 239203 | Sc RFC[2+3+4]-pET | Overexpress Sc RFC2, RFC3, & RFC4 in E. coli |
| 239204 | Sc Rad27-pET24 | Overexpress Sc Rad27 (FEN1) in E. coli |
| 239205 | Sc Rad27-S6- pET24 | Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli |
| 239471 | Sc FLAG-S6-PCNA-pET | Overexpress Sc FLAG-S6-PCNA in E. coli |
| 239472 | Sc FLAG-S6-RFC1-pRSFDuet | Overexpress Sc FLAG-S6-RFC1 in E. coli |
| 239473 | Sc FLAG-S6-RFC1-pRS405/GAL-L | Overexpress Sc FLAG-S6-RFC1 in yeast (integrated) |
| 239474 | Sc RFC[2+5]-pRS403/GAL | Overexpress Sc RFC2 & RFC5 in yeast (integrated) |
| 239475 | Sc RFC[3+4]-pRS402/GAL | Overexpress Sc RFC3 & RFC4 in yeast (integrated) |
| 239476 | Sc FLAG-RFC1-pRS405/GAL-L | Overexpress Sc FLAG-RFC1 in yeast (integrated) |
| 240732 | Sc [rad24 + RFC5]-pLANT/RIL | Over-express Sc rad24 & Sc RFC5 in E. coli |
| 240733 | Sc FLAG-rad24-pRSFDuet | Over-express FLAG-rad24 in E. coli |
| 240734 | Sc FLAG-S6-rad24-pRSFDuet | Over-express FLAG-S6-rad24 in E. coli |
| 240735 | Sc FLAG-rad24-pRS404/GAL-L | Over-express FLAG-rad24 in yeast (integrated) |
| 240736 | Sc FLAG-S6-rad24- pRS404/GAL-L | Over-express FLAG-S6-rad24 in yeast (integrated) |
| 240737 | Sc mec1-S6-P/FLAG-pRS403/GAL-L | Over-express Sc mec1-S6-PreScission Protease Site-FLAG in yeast (integrated) |
| 240738 | Sc ddc2-pRS404/GAL-U | Over-express Sc ddc2 in yeast (integrated) |
| 240739 | Sc dpb11-FLAG-pRS405/GAL | Over-express Sc dpb11-FLAG in Yeast (integrated) |
| 240740 | Sc [ddc1 + rad17]-pET | Over-express Sc ddc1 & Sc rad17 in E. coli |
| 240741 | Sc FLAG-kin-mec3-pRSFDuet | Over-express Sc FLAG-PKA kinase motif-mec3 in E. coli |
| 240742 | Sc RFA[1+2]-pET | Over-express Sc RFA1 & RFA2 in E. coli |
| 240743 | Sc hk/P-RFA3-pCDFDuet | Over-express his(10)-PKA kinase motif-PreScission protease site-Sc RFA3 in E. coli |
| 241258 | Sc rev3-FLAG-pRS402/GAL | Over-express Sc rev3-FLAG (Sc Pol zeta subunit) in yeast (integrated) |
| 241259 | Sc rev7-pRS405/GAL | Over-express Sc rev7 (Sc Pol zeta subunit) in yeast (integrated) |
| 241260 | Sc FLAG-rad30-pRS403/GAL | Over-express Sc FLAG-rad30 (Sc Pol eta) in yeast (integrated) |
| 241261 | Sc FLAG-POL4-pRS404/GAL-L | Over-express Sc FLAG-POL4 in yeast (integrated) |
| 241262 | Ec dnaE-pET(3c) | Over-express E. coli Pol III subunit alpha in E. coli |
| 241263 | Ec dnaQ-pCDFDuet | Over-express E. coli Pol III subunit epsilon in E. coli |
| 241264 | Ec holE-pCDFDuet | Over-express E. coli Pol III subunit theta in E. coli |
| 241265 | Ec (gamma-only)-pET(11a)(ACH–) | Over-express E. coli Pol III subunit gamma-only in E. coli |
| 241266 | Ec dnaB-pET | Over-express Ec dnaB subunit in E. coli |
| 241267 | Ec FLAG-dnaB-pET | Over-express Ec FLAG-dnaB subunit in E. coli |
| 241268 | Ec FLAG-S6-dnaB-pET | Over-express Ec FLAG-S6-dnaB subunit in E. coli |
| 241269 | Ec dnaC-pET(11a) | Over-express Ec dnaC subunit in E. coli |
| 241975 | Sc Pol1-FLAG-pRS402/GAL-L | Over-express Sc Pol1-FLAG (Sc pol alpha - large subunit) in yeast (integrated) |
| 241976 | Sc Pol12-pRS406/GAL | Over-express Sc Pol12 (Sc pol alpha subunit) in yeast (integrated) |
| 241977 | Sc [Pri1 + Pri2]-pRS403/GAL | Over-express Sc Pri1 & Pri2 (Sc pol alpha subunits) in yeast (integrated) |
| 241978 | Sc FLAG-Pol2-pRS425/GAL | Over-express Sc FLAG-Pol2 (Pol epsilon large subunit) in yeast (episomal) |
| 241979 | Sc FLAG-Rev1-pRS425/GAL | Over-express Sc FLAG-Rev1 in yeast (episomal) |
| 241980 | Sc FLAG-Rev1-pRS404/GAL | Over-express Sc FLAG-Rev1 in yeast (integrated) |